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한국생명공학연구원

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J. Hazard. Mater., 2023 Jun 15;452:131223 | https://doi.org/10.1016/j.jhazmat.2023.131223 Differential particle and ion kinetics of silver nanoparticles in the lungs and biotransformation to insoluble silver sulfide

Soyeon Jeon ^a,1, Wang Sik Lee ^b,1, Kyung Seuk Song ^c, Jiyoung Jeong ^a, Sinuk Lee ^a, Songyeon Kim ^a, Gyuri Kim^a, Ji-Su Kim ^d, Jinyoung Jeong ^b,e, Wan-Seob Cho ^a

^aLab of Toxicology, Department of Health Sciences, The Graduate School of Dong-A University, 37, Nakdong-daero 550 beon-gil, Saha-gu, Busan 49315, the Republic of Korea
^bEnvironmental Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon 34141, the Republic of Korea
^cKorea Conformity Laboratories, 8, Gaetbeol-ro 145 beon-gil, Yeonsu-gu, Incheon 21999, the Republic of Korea
^dPrimate Resources Center (PRC), Korea Research Institute of Bioscience and Biotechnology (KRIBB), Jeongeup 56216, the Republic of Korea
^eKRIBB School, University of Science and Technology, 217 Gajeong-ro, Yuseong-gu, Daejeon 34113, the Republic of Korea

¹S.J. and W.S.L. equally contributed to this work.
Corresponding authors: Jinyoung Jeong, Wan-Seob Cho

Abstract

The measurement of nanoparticles (NPs) in a biological matrix is essential in various toxicity studies. However, the current knowledge has limitations in differentiating particulate and ionic forms and further identification of their biotransformation. Herein, we evaluate the biotransformation and differential lung clearance kinetics of particulate and ionic forms using PEGylated silver NPs (AgNP-PEGs; 47.51 nm) and PEGylated gold NPs (AuNP-PEGs; 11.76 nm). At 0, 3, and 6 h and 1, 3, 7, and 14 days after a single pharyngeal aspiration in mice at 25 μg/mouse, half of the lung is digested by proteinase K (PK) to separate particulates and ions, and the other half is subjected to the acid digestion method for comparison. The quantitative and qualitative evaluation of lung clearance kinetics suggests that AgNP-PEGs are quickly dissolved and transformed into insoluble silver sulfide (Ag₂S), which shows a fast-clearing early phase (0 -6 h; particle T_1/2: 4.8 h) and slow-clearing late phase (1 -14 days; particle T_1/2: 13.20 days). In contrast, AuNP-PEGs were scarcely cleared or biotransformed in the lungs for 14 days. The lung clearance kinetics of AgNPs and biotransformation shown in this study can be informed by the PK digestion method and cannot be obtained using the acid digestion method.

논문정보

형식Research article
게재일2023년 06월 (BRIC 등록일 2023-06-19)
연구진국내 연구진
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