정윤 (University of Illinois Urbana-Champaign) | 한빛사논문 > 한빛사

한빛사논문

조회334

University of Illinois Urbana-Champaign

CV File 435 kb

J. Hazard. Mater., Available online 22 May 2023, 131672 | https://doi.org/10.1016/j.jhazmat.2023.131672 Toxicity of per- and polyfluoroalkyl substances to microorganisms in confined hydrogel structures

Yoon Jeong ^a,b,c, Khushali Vyas ^d, Joseph Irudayaraj ^a,b,c,e

^aDepartment of Bioengineering, University of Illinois Urbana-Champaign, Urbana, Illinois, USA
^bCancer Center at Illinois, University of Illinois Urbana-Champaign, Urbana, Illinois, USA
^cBiomedical Research Center, Mills Breast Cancer Institute, Carle Foundation Hospital, Urbana, Illinois, USA
^dSchool of Molecular and Cellular Biology, University of Illinois Urbana-Champaign, Urbana, Illinois, USA
^eCarle R. Woese Institute for Genomic Biology, Beckman Institute, Holonyak Micro and Nanotechnology Laboratory, Urbana, Illinois, USA

Corresponding author : Joseph Irudayaraj

Abstract

Per- and polyfluoroalkyl substances (PFAS) as a group of environmentally persistent synthetic chemicals has been widely used in industrial and consumer products. Bioaccumulation studies have documented the adverse effects of PFAS in various living organisms. Despite the large number of studies, experimental approaches exist to evaluate the toxicity of PFAS on bacteria in a biofilm-like niche as structured microbial communities are sparse. This study suggests a facile approach to querry the toxicity of PFOS and PFOA on bacteria (Escherichia coli strain K12 MG1655 strain) in a biofilm-like niche provided by hydrogel-based core-shell beads. Our study shows that E. coli MG1655 upon complete confinement in hydrogel beads exhibit altered physiological characteristics of viability, biomass, and protein expression, compared to their susceptible counterpart cultivated under planktonic conditions. We find that soft-hydrogel engineering platforms may provide a protective role for microorganisms from environmental contaminants, depending on the size or thickness of the protective/barrier layer. We expect our study to provide insights on the toxicity of environmental contaminants on organisms under encapsulated growth conditions that could potentially be useful for toxicity screening and in evaluating ecological risk of soil, plant, and mammalian microbiome in the long-term.

논문정보

형식Research article
게재일2023년 05월 (BRIC 등록일 2023-06-02)
연구진국외 연구진
분야 생명과학 > 생물공학

댓글 작성 로그인

CV 열람하기

연락처 보기