봉세랑 (서강대학교)

한빛사논문

조회454

봉세랑Serang Bong

서강대학교

Nucleic Acids Res., 2023 May 9;gkad340 | https://doi.org/10.1093/nar/gkad340 AT-specific DNA visualization revisits the directionality of bacteriophage λ DNA ejection

Serang Bong^1,†, Chung Bin Park^1,†, Shin-Gyu Cho^2,†, Jaeyoung Bae^1,†, Natalia Diyah Hapsari^1,3,†, Xuelin Jin^1,4,†, Sujung Heo¹, Ji-eun Lee², Kaori Hashiya⁵, Toshikazu Bando⁵, Hiroshi Sugiyama ⁵, Kwang-Hwan Jung ^2,*, Bong June Sung^1,* and Kyubong Jo^1,*

¹Department of Chemistry, Sogang University, Seoul 04107, Korea,
²Department of Life Science, Sogang University, Seoul 04107, Korea,
³Chemistry Education Program, Department of Mathematics and Science Education, Sanata Dharma University, Yogyakarta 55282, Indonesia,
⁴College of Agriculture, Yanbian University, Yanji133000, China
⁵Department of Chemistry, Graduate School of Science, Kyoto University, Sakyo-Ku, Kyoto606-8502, Japan

^†The authors wish it to be known that, in their opinion, the first six authors should be regarded as Joint First Authors.
^*Correspondence : Kwang-Hwan Jung, Bong June Sung, Kyubong Jo

Abstract

In this study, we specifically visualized DNA molecules at their AT base pairs after in vitro phage ejection. Our AT-specific visualization revealed that either end of the DNA molecule could be ejected first with a nearly 50% probability. This observation challenges the generally accepted theory of Last In First Out (LIFO), which states that the end of the phage λ DNA that enters the capsid last during phage packaging is the first to be ejected, and that both ends of the DNA are unable to move within the extremely condensed phage capsid. To support our observations, we conducted computer simulations that revealed that both ends of the DNA molecule are randomized, resulting in the observed near 50% probability. Additionally, we found that the length of the ejected DNA by LIFO was consistently longer than that by First In First Out (FIFO) during in vitro phage ejection. Our simulations attributed this difference in length to the stiffness difference of the remaining DNA within the phage capsid. In conclusion, this study demonstrates that a DNA molecule within an extremely dense phage capsid exhibits a degree of mobility, allowing it to switch ends during ejection.

논문정보

형식Research article
게재일2023년 05월 (BRIC 등록일 2023-05-24)
연구진국내(교신)+국외 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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