한빛사논문
- 조회510
Junghoon Park 1,5, Axel J. Giudicatti 2,5, Zein Eddin Bader 1,5, Min Kyun Han 3,5 , Christian
Møller 3, Agustin L. Arce 2, Zheng-Yi Xu 4, Seong Wook Yang 3, *, Pablo A. Manavella 2,* , DaeJin Yun 1,4,*
1Department of Biomedical Science and Engineering, Konkuk University, Seoul, South Korea.
2Instituto de Agrobiotecnología del Litoral (CONICET-UNL), Cátedra de Biología Celular y Molecular,Facultad de Bioquímica y Ciencias Biológicas, Universidad Nacional del Litoral, 3000 Santa Fe, Argentina.
3Department of Systems Biology, Institute of Life Science and Biotechnology, Yonsei University, Seoul 14 120-749, Korea.
4Key Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), Northeast Normal University, Changchun, China.
5These authors contributed equally.
*Corresponding authors: Correspondence to Seong Wook Yang, Pablo A. Manavella, DaeJin Yun
Abstract
The regulation of microRNA (miRNA) biogenesis is crucial for maintaining plant homeostasis under biotic and abiotic stress. The crosstalk between the RNA polymerase II (Pol-II) complex and the miRNA processing machinery has emerged as a central hub modulating transcription and co-transcriptional processing of primary miRNA transcripts (pri-miRNAs). However, it remains unclear how miRNA-specific transcriptional regulators recognize MIRNA loci. Here, we show that the Arabidopsis (Arabidopsis thaliana) HIGH EXPRESSION OF OSMOTICALLY RESPONSIVE GENE15 (HOS15)-HISTONE DEACETYLASE9 (HDA9) complex is a conditional suppressor of miRNA biogenesis, particularly in response to ABA. When treated with ABA, hos15/hda9 mutants show enhanced transcription of pri-miRNAs that is accompanied by increased processing, leading to over-accumulation of a set of mature miRNAs. Moreover, upon recognition of the nascent pri-miRNAs, the ABA-induced recruitment of the HOS15-HDA9 complex to MIRNA loci is guided by HYPONASTIC LEAVES 1 (HYL1). The HYL1-dependent recruitment of the HOS15-HDA9 complex to MIRNA loci suppresses expression of MIRNAs and processing of pri-miRNA. Most importantly, our findings indicate that nascent pri-miRNAs serve as scaffolds for recruiting transcriptional regulators, specifically to MIRNA loci. This indicates that RNA molecules can act as regulators of their own expression by causing a negative feedback loop that turns off their transcription, providing a self-buffering system.
논문정보
- Research article
- 2023년 05월 (BRIC 등록일 2023-05-24)
- 국내(교신)+국외 연구진
- 생명과학 > 식물학
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