한빛사논문
- 조회590
Min-Seok Oh 1,2, Seul-Gi Lee 1, Gwan-Ho Lee 2, C-Yoon Kim 3, Jong Han Song 2, Byung-Yong Yu 2, Hyung Min Chung 1,4
1Department of Stem Cell Biology, School of Medicine, Konkuk University, 120 Neungdong-Ro, Gwangjin-Gu, Seoul, 05029, Republic of Korea.
2Advanced Analysis and Data Center, Korea Institute of Science and Technology, Hwarang-ro 14-gil 5, Seongbuk-gu, Seoul, 02792, Republic of Korea.
3College of Veterinary Medicine, Konkuk University, Gwangjin-Gu, Seoul, 05029, Republic of Korea.
4Mirae Cell Bio Co. Ltd, Seoul, 04795, Republic of Korea.
M.S.O. and S.G.L., and B.Y.Y. and H.M.C. contributed equally to this work as first and corresponding authors, respectively.
CORRESPONDING AUTHORS : Byung-Yong Yu, Hyung Min Chung
Abstract
As the potential of pluripotent stem cell-derived differentiated cells has been demonstrated in regenerative medicine, differentiated vascular endothelial cells (ECs) are emerging as a therapeutic agent for the cardiovascular system. To verify the therapeutic efficacy of differentiated ECs in an ischemic model, human embryonic stem cells (hESCs) are induced as EC lineage and produce high-purity ECs through fluorescence-activated cell sorting (FACS). When hESC-ECs are transplanted into a hindlimb ischemic model, it is confirmed that blood flow and muscle regeneration are further improved by creating new blood vessels together with autologous ECs than the primary cell as cord blood endothelial progenitor cells (CB-EPCs). In addition, previously reported studies show the detection of transplanted cells engrafted in blood vessels through various tracking methods, but fail to provide accurate quantitative values over time. In this study, it is demonstrated that hESC-ECs are engrafted approximately sevenfold more than CB-EPCs by using an accelerator mass spectrometry (AMS)-based cell tracking technology that can perform quantification at the single cell level. An accurate quantification index is suggested. It has never been reported in in vivo kinetics of hESC-ECs that can act as therapeutic agents.
논문정보
- Research article
- 2023년 04월 (BRIC 등록일 2023-05-23)
- 국내 연구진
- 바이오·의료융합 > 의료기기
댓글 작성 로그인
팝업 닫기관련 링크
연구자 ID
관련분야 연구자보기
관련분야 논문보기
