한빛사논문
- 조회564
Byung Seok Cha 1, Young Jun Jang 1, Eun Sung Lee 1, Do Yeon Kim 1, Ji Su Woo 1, Jinseo Son 1, Seokjoon Kim 1, Jiye Shin 1, Jinjoo Han 1, Seokhwan Kim 1, Ki Soo Park 1
1Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, 05029, Republic of Korea.
B.S.C., Y.J.J., and E.S.L. contributed equally to this work.
CORRESPONDING AUTHOR : Ki Soo Park
Abstract
Colorectal cancer (CRC) as the second leading cause of global cancer deaths poses critical challenges in clinical settings. Cancer-derived small extracellular vesicles (sEVs), which are secreted by cancer cells, have been shown to mediate tumor development, invasion, and even metastasis, and have thus received increasing attention for the development of cancer diagnostic or therapeutic platforms. In the present study, the sEV-targeted systematic evolution of ligands by exponential enrichment (E-SELEX) is developed to generate a high-quality aptamer (CCE-10F) that recognizes and binds to CRC-derived sEVs. Via an in-depth investigation, it is confirmed that this novel aptamer possesses high affinity (Kd = 3.41 nm) for CRC-derived sEVs and exhibits a wide linear range (2.0 × 104 -1.0 × 106 particles µL-1 ) with a limit of detection (LOD) of 1.0 × 103 particles µL-1 . Furthermore, the aptamer discriminates CRC cell-derived sEVs from those derived from normal colon cell, human serum, and other cancer cells, showing high specificity for CRC cell-derived sEVs and significantly suppresses the critical processes of metastasis, including cellular migration, invasion, and angiogenesis, which are originally induced by sEVs themselves. These findings are highly encouraging for the potential use of the aptamer in sEV-based diagnostic and therapeutic applications.
논문정보
- Research article
- 2023년 05월 (BRIC 등록일 2023-05-22)
- 국내 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
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