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박은경Eungyeong Park

강원대학교

Biosens. Bioelectron., Available online 29 April 2023 | https://doi.org/10.1016/j.bios.2023.115366 Highly selective and quantitative in situ monitoring of cell surface proteins by SERS immunoassay system

Eungyeong Park ^a,1, Wijin Kim^b,1, Shuang Guo ^a, Sila Jin ^c, Yeonju Park ^c, Jongmin Park^a, Hyuk Sang Yoo ^b,c, Ju Hyun Park ^b, Young Mee Jung^a,c

^aDepartment of Chemistry, Institute for Molecular Science and Fusion Technology, Kangwon National University, Chuncheon-si, Gangwon-do, 24341, Republic of Korea
^bDepartment of Biomedical Science, Kangwon National University, Chuncheon-si, Gangwon-do, 24341, Republic of Korea
^cKangwon Radiation Convergence Research Support Center, Kangwon National University, Chuncheon-si, Gangwon-do, 24341, Republic of Korea

¹These authors contributed equally.
Corresponding authors: Ju Hyun Park, Young Mee Jung

Abstract

Due to their pivotal roles in many biological functions, cell surface proteins (CSPs) are often used for cancer prognosis, as evidenced by a number of studies that have reported significant changes in the expression levels of specific surface proteins depending on the stage of tumorigenesis and selection/variety of reprogrammed cells during cell fate conversion. Current CSP detection strategies suffer from poor selectivity and lack the ability for in situ analysis but maintain the spatial information between cells. Here, we have fabricated nanoprobes for surface-enhanced Raman scattering (SERS) immunoassays by conjugating a specific antibody onto silica-coated gold nanoparticles incorporating an individual Raman reporter (Au-tag@SiO₂-Ab NPs) for highly sensitive and selective in situ detection in different types of cells. When multiple HEK293 cell lines stably expressing different levels of the CSP, ACE2, were investigated by the SERS immunoassay, we demonstrated that the level of ACE2 expression in each cell line could be statistically distinguished from that in the other cell lines, indicating the quantitative feature of this biosensing system. When detecting living cells without cell lysis or fixation, as well as fixed cells, the levels of the epithelial CSPs, EpCAM (epithelial cell adhesion molecule) and E-cadherin, were successfully determined using our Au-tag@SiO₂-Ab NPs and SERS immunoassay system in a highly selective and quantitative manner without significant cytotoxicity. Hence, our work provides technical insight into the development of a biosensing platform for a variety of biomedical applications, such as cancer metastasis prognosis and the in situ monitoring of stem cell reprogramming and differentiation.

논문정보

형식Research article
게재일2023년 04월 (BRIC 등록일 2023-05-19)
연구진국내 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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