Olatunji Ajiteru (한림대학교 의과대학) | 한빛사논문 > 한빛사

한빛사논문

조회269

한림대학교 의과대학

Acta Biomater., 2023 Apr 28;S1742-7061(23)00234-9 | https://doi.org/10.1016/j.actbio.2023.04.034 A digital light processing 3D-printed artificial skin model and full-thickness wound models using silk fibroin bioink

Kyu Young Choi ^a,1, Olatunji Ajiteru ^b,1, Heesun Hong ^b, Ye Ji Suh ^b, Md Tipu Sultan ^b, Hanna Lee ^b, Ji Seung Lee ^b, Young Jin Lee ^b, Ok Joo Lee ^b, Soon Hee Kim ^b, Chan Hum Park^b,c

^aDepartment of Otorhinolaryngology-Head and Neck Surgery, Hallym University Kangnam Sacred Heart Hospital, Seoul 07441, Republic of Korea
^bNano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, 1 Hallymdaehak-gil, Chuncheon, Gangwon-do 24252, Republic of Korea
^cDepartment of Otorhinolaryngology-Head and Neck Surgery, Chuncheon Sacred Heart Hospital, School of Medicine, Hallym University, Chuncheon 24252, Republic of Korea

¹These authors contributed equally to this work.
Corresponding author : Chan Hum Park

Abstract

A three-dimensional (3D) artificial skin model offers diverse platforms for skin transplantation, disease mechanisms, and biomaterial testing for skin tissue. However, implementing physiological complexes such as the neurovascular system with living cells in this stratified structure is extremely difficult. In this study, full-thickness skin models were fabricated from methacrylated silk fibroin (Silk-GMA) and gelatin (Gel-GMA) seeded with keratinocytes, fibroblasts, and vascular endothelial cells representing the epidermis and dermis layers through a digital light processing (DLP) 3D printer. Printability, mechanical properties, and cell viability of the skin hydrogels fabricated with different concentrations of Silk-GMA and Gel-GMA were analyzed to find the optimal concentrations for the 3D printing of the artificial skin model. After the skin model was DLP-3D printed using Gel-GMA 15% + Silk-GMA 5% bioink, cultured, and air-lifted for four weeks, well-proliferated keratinocytes and fibroblasts were observed in histological analysis, and increased expressions of Cytokeratin 13, Phalloidin, and CD31 were noted in immunofluorescence staining. Furthermore, full-thickness skin wound models were 3D-printed to evaluate the wound-healing capabilities of the skin hydrogel. When the epidermal growth factor (EGF) was applied, enhanced wound healing in the epidermis and dermis layer with the proliferation of keratinocytes and fibroblasts was observed. Also, the semi-quantitative reverse transcription-polymerase chain reaction revealed increased expression of Cytokeratin 13, fibroblast growth factor, and CD31 in the EGF-treated group relative to the control group. The DLP 3D-printed artificial skin model was mechanically stable and biocompatible for more than four weeks, demonstrating the potential for application in skin tissue engineering.

논문정보

형식Research article
게재일2023년 04월 (BRIC 등록일 2023-05-18)
연구진국내 연구진
분야 의약학 > 응용의학

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