한빛사논문
- 조회455
Liang Hu 1,2†, Xiaoling Liu 1,2†, Qiaolan Zheng 2, Wuhe Chen 2, Hao Xu 1,2, Hengrui Li 1,2, Jiaxin Luo 1,2, Rui Yang 1,2, Xulong Mao 3, Siyao Wang 1,2, Tucan Chen 1,2, Luke P Lee 4,5,6,7, Fei Liu 1,2
1National Engineering Research Center of Ophthalmology and Optometry, Eye Hospital, Wenzhou Medical University, Wenzhou 325027, China.
2National Clinical Research Center for Ocular Diseases, Eye Hospital, Wenzhou Medical University, Wenzhou 325027, China.
3The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China.
4Renal Division and Division of Engineering in Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02115, USA.
5Department of Bioengineering, University of California at Berkeley, Berkeley, CA 94720, USA.
6Department of Electrical Engineering and Computer Science, University of California at Berkeley, Berkeley, CA 94720, USA.
7Institute of Quantum Biophysics, Department of Biophysics, Sungkyunkwan University, Suwon, Gyeonggi-do 16419, Korea.
†These authors contributed equally to this work.
*Corresponding authors: Luke P. Lee, Fei Liu
Abstract
Discovering the secrets of diseases from tear extracellular vesicles (EVs) is well-recognized and appreciated. However, a precise understanding of the interaction network between EV populations and their biogenesis from our body requires more in-depth and systematic analysis. Here, we report the biological profiles of different-size tear EV subsets from healthy individuals and the origins of EV proteins. We have identified about 1800 proteins and revealed the preferential differences in the biogenesis among distinct subsets. We observe that eye-related proteins that maintain retinal homeostasis and regulate inflammation are preferentially enriched in medium-size EVs (100 to 200 nm) fractions. Using universal analysis in combination with the Human Protein Atlas consensus dataset, we found the genesis of tear EV proteins with 37 tissues and 79 cell types. The proteins related to retinal neuronal cells, glial cells, and blood and immune cells are selectively enriched among EV subsets. Our studies in heterogeneous tear EVs provide building blocks for future transformative precision molecular diagnostics and therapeutics.
논문정보
- Research article
- 2023년 03월 (BRIC 등록일 2023-05-15)
- 국내(교신)+국외 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
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