백승필 (고려대학교) | 한빛사논문 > 한빛사

한빛사논문

조회576

고려대학교

CV File 567 kb

Biosens. Bioelectron., 2023 May 15;228:115202 | https://doi.org/10.1016/j.bios.2023.115202 Ultra-sensitive label-free SERS biosensor with high-throughput screened DNA aptamer for universal detection of SARS-CoV-2 variants from clinical samples

Ki Sung Park ^a, Anna Choi ^a, Hyun Jung Kim ^a,b, Insu Park ^a,c, Mi-Suk Eom ^d, Sang-Gu Yeo ^d, Ryeo Gang Son ^a, Tae-In Park ^a, Gyudo Lee^a, Hyongsok Tom Soh ^e,f, Yoochan Hong ^b, Seung Pil Pack^a

^aDepartment of Biotechnology and Bioinformatics, Korea University, Sejong, 30019, Republic of Korea
^bDepartment of Medical Device, Korea Institute of Machinery and Materials (KIMM), Daegu, 42994, Republic of Korea
^cDepartment of Biomedical Engineering, Konyang University, Daejeon, 35365, Republic of Korea
^dDivision of Infectious Diseases, Sejong Institute of Health & Environment, Sejong, 30015, Republic of Korea
^eDepartment of Electrical Engineering, Stanford University, Stanford, CA, 94305, USA
^fDepartment of Radiology, Stanford University, Stanford, CA, 94305, USA

Corresponding authors: Yoochan Hong, Seung Pil Pack

Abstract

COVID-19, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has caused an ongoing global pandemic with economic and social disruption. Moreover, the virus has persistently and rapidly evolved into novel lineages with mutations. The most effective strategy to control the pandemic is suppressing virus spread through early detection of infections. Therefore, developing a rapid, accurate, easy-to-use diagnostic platform against SARS-CoV-2 variants of concern remains necessary. Here, we developed an ultra-sensitive label-free surface-enhanced Raman scattering-based aptasensor as a countermeasure for the universal detection of SARS-CoV-2 variants of concern. In this aptasensor platform, we discovered two DNA aptamers that enable binding to SARS-CoV-2 spike protein via the Particle Display, a high-throughput screening approach. These showed high affinity that exhibited dissociation constants of 1.47 ± 0.30 nM and 1.81 ± 0.39 nM. We designed a combination with the aptamers and silver nanoforest for developing an ultra-sensitive SERS platform and achieved an attomolar (10^-18 M) level detection limit with a recombinant trimeric spike protein. Furthermore, using the intrinsic properties of the aptamer signal, we demonstrated a label-free aptasensor approach, enabling use without the Raman tag. Finally, our label-free SERS-combined aptasensor succeeded in detecting SARS-CoV-2 with excellent accuracy, even in clinical samples with variants of concern, including the wild-type, delta, and omicron variants.

논문정보

형식Research article
게재일2023년 05월 (BRIC 등록일 2023-05-12)
연구진국내(교신)+국외 연구진
분야 생명과학 > 생물공학

댓글 작성 로그인

CV 열람하기

연락처 보기