한빛사논문
- 조회280
연세대학교
Yoon Ho Roh 1,2, Chang Yeol Lee 1,3, Sujin Lee 1,2, Hyunho Kim 3,4, Amy Ly 5, Cesar M Castro 3,6, Jinwoo Cheon 1,2,7, Jae-Hyun Lee 1,2, Hakho Lee 1,2,3,4
1Institute for Basic Science (IBS), Center for Nanomedicine, Seoul, 03722, Republic of Korea.
2Graduate Program of Nano Biomedical Engineering (NanoBME), Advanced Science Institute Yonsei University, Seoul, 03722, Republic of Korea.
3Center for Systems Biology, Massachusetts General Hospital Research Institute, Boston, MA, 02114, USA.
4Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, 02114, USA.
5Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, 02114, USA.
6Department of Medicine, Massachusetts General Hospital, Harvard Medical School, Boston, MA, 02114, USA.
7Department of Chemistry, Yonsei University, Seoul, 03722, Republic of Korea.
CORRESPONDING AUTHORS : Jae-Hyun Lee, Hakho Lee
Abstract
CRISPR/Cas systems offer a powerful sensing mechanism to transduce sequence-specific information into amplified analytical signals. However, performing multiplexed CRISPR/Cas assays remains challenging and often requires complex approaches for multiplexed assays. Here, a hydrogel-based CRISPR/Cas12 system termed CLAMP (Cas-Loaded Annotated Micro-Particles) is described. The approach compartmentalizes the CRISPR/Cas reaction in spatially-encoded hydrogel microparticles (HMPs). Each HMP is identifiable by its face code and becomes fluorescent when target DNA is present. The assay is further streamlined by capturing HMPs inside a microfluidic device; the captured particles are then automatically recognized by a machine-learning algorithm. The CLAMP assay is fast, highly sensitive (attomolar detection limits with preamplification), and capable of multiplexing in a single-pot assay. As a proof-of-concept clinical application, CLAMP is applied to detect nucleic acid targets of human papillomavirus in cervical brushing samples.
논문정보
- Research article
- 2023년 04월 (BRIC 등록일 2023-05-30)
- 국내(교신)+국외 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
댓글 작성 로그인
팝업 닫기관련 링크
연구자 키워드
연구자 ID
관련분야 연구자보기
소속기관 논문보기
관련분야 논문보기
