한빛사논문
- 조회416
Jae Sung Cho1,2,5,8, Dongsoo Yang1,2,6,8, Cindy Pricilia Surya Prabowo1,2, Mohammad Rifqi Ghiffary2,3, Taehee Han1,2, Kyeong Rok Choi1,2, Cheon Woo Moon1,2, Hengrui Zhou1,2, Jae Yong Ryu1,2,7, Hyun Uk Kim2,3,4 & Sang Yup Lee1,2,4
1Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 four), Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST), Daejeon 34141, Republic of Korea.
2Systems Metabolic Engineering and Systems Healthcare Cross-Generation Collaborative Laboratory, KAIST, Daejeon 34141, Republic of Korea.
3Systems Biology and Medicine Laboratory, Department of Chemical and Biomolecular Engineering (BK21 four), KAIST, Daejeon 34141, Republic of Korea.
4KAIST Institute for Artificial Intelligence, BioProcess Engineering Research Center and BioInformatics Research Center, KAIST, Daejeon 34141, Republic of Korea.
5Present address: Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA, USA.
6Present address: Department of Chemical and Biological Engineering, Korea University, Seoul 02481, Republic of Korea.
7Present address: Department of Biotechnology, College of Science and Technology, Duksung Women’s University, Seoul, Republic of Korea.
8These authors contributed equally: Jae Sung Cho, Dongsoo Yang.
Corresponding author : Correspondence to Sang Yup Lee.
Abstract
Synthetic sRNAs allow knockdown of target genes at translational level, but have been restricted to a limited number of bacteria. Here, we report the development of a broad-host-range synthetic sRNA (BHR-sRNA) platform employing the RoxS scaffold and the Hfq chaperone from Bacillus subtilis. BHR-sRNA is tested in 16 bacterial species including commensal, probiotic, pathogenic, and industrial bacteria, with >50% of target gene knockdown achieved in 12 bacterial species. For medical applications, virulence factors in Staphylococcus epidermidis and Klebsiella pneumoniae are knocked down to mitigate their virulence-associated phenotypes. For metabolic engineering applications, high performance Corynebacterium glutamicum strains capable of producing valerolactam (bulk chemical) and methyl anthranilate (fine chemical) are developed by combinatorial knockdown of target genes. A genome-scale sRNA library covering 2959 C. glutamicum genes is constructed for high-throughput colorimetric screening of indigoidine (natural colorant) overproducers. The BHR-sRNA platform will expedite engineering of diverse bacteria of both industrial and medical interest.
논문정보
- Research article
- 2023년 04월 (BRIC 등록일 2023-04-26)
- 국내(교신)+국외 연구진
- 생명과학 > 응용생물화학
관련 보도자료
- Bio통신원
- 2023. 05. 10
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