한빛사논문
- 조회422
Sung Eun Seo 1,2, Seong Gi Lim 1,2, Kyung Ho Kim 1,2, Jinyeong Kim 1, Chan Jae Shin 2, Soomin Kim 1, Lina Kim 1, Seung Hwan Lee 3,4, Song Yee Jang 5,6, Hyun Woo Oh 5,6, Hyang-Ae Lee 7, Woo-Keun Kim 7, Yoo Min Park 8, Kyoung G Lee 8, Sang Hun Lee 4, Siyoung Ha 6, Oh Seok Kwon 1,2
1Infectious Disease Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, South Korea.
2Department of Nano Engineering, SKKU Advanced Institute of Nanotechnology (SAINT), Sungkyunkwan University (SKKU), 2066 Seobu-ro, Jangan-gu, Suwon, 16419, South Korea.
3Department of Bionano Engineering, Center for Bionano Intelligence Education and Research, Hanyang University, 55 Hanyangdaehak-ro, Sangnok-gu, Ansan, 15588, South Korea.
4Department of Chemical and Biological Engineering, Hanbat National University, 125 Dongseo-daero, Yuseong-gu, Daejeon, 34158, South Korea.
5Core Research Facility & Analysis Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 125 Gwahak-ro, Yuseong-gu, Daejeon, 34141, South Korea.
6School of Pharmacy, University of Maryland Eastern Shore, Princess Anne, MD, 21853, United States.
7Department of Predictive Toxicology, Korea Institute of Toxicology, 141 Gajeong-ro, Yuseong-gu, Daejeon, 34114, South Korea.
8Center for NanoBio Development, National NanoFab Center, 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, South Korea.
CORRESPONDING AUTHORS: Sang Hun Lee, Siyoung Ha, Oh Seok Kwon
Abstract
The sense of spiciness is related to the stimulation of vanilloid compounds contained in the foods. Although, the spiciness is commonly considered as the part of taste, it is more classified to the sense of pain stimulated on a tongue, namely, pungency, which is described as a tingling or burning on the tongue. Herein, first, a reusable electronic tongue based on a transient receptor potential vanilloid 1 (TRPV1) nanodisc conjugated graphene field-effect transistor is fabricated and spiciness-related pain evaluation with reusable electrode is demonstrated. The pungent compound reactive receptor TRPV1 is synthesized in the form of nanodiscs to maintain stability and reusability. The newly developed platform shows highly selective and sensitive performance toward each spiciness related vanilloid compound repeatably: 1 aM capsaicin, 10 aM dihydrocapsaicin, 1 fM piperine, 10 nM allicin, and 1 pM AITC. The binding mechanism is also examined by simulation. Furthermore, the elimination of the burning sensation on the tongue after eating spicy foods is not investigated. Based on the synthesis of micelles composed of casein protein (which is contained in skim milk) that remove pungent compounds bound to TRPV1 nanodisc, the deactivation of TRPV1 is investigated, and the electrode is reusable that mimics electronic tongue.
논문정보
- Research article
- 2023년 03월 (BRIC 등록일 2023-04-26)
- 국내(교신)+국외 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
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