한빛사논문
- 조회402
Mohan Pei a,1, Hanjun Hwangbo a,b,1, GeunHyung Kim a,b,c
aDepartment of Precision Medicine, Sungkyunkwan University School of Medicine (SKKU-SOM), Suwon, 16419, Republic of Korea
bInstitute of Quantum Biophysics, Department of Biophysics, Sungkyunkwan University, Suwon, Gyeonggi-do, 16419, Republic of Korea
cBiomedical Institute for Convergence at SKKU (BICS), Sungkyunkwan University, Suwon, 16419, Republic of Korea
1These authors contributed equally to this work.
Corresponding author: GeunHyung Kim
Abstract
Hierarchical scaffolds have been extensively used in various tissue engineering applications as they can provide unique biofunctional properties, including biophysical cues, to successfully accomplish preferred cell attachment, growth, and differentiation depending on the target tissues. In this study, we propose a new strategy for fabricating hierarchical structures consisting of microfibrous collagen/poly(ε-caprolactone) (PCL) bundles obtained using a 3D printing process supplemented with a unique bioink, plasma treatment, and collagen coating process. To obtain the hierarchical fibrous PCL structure, we used an alginate matrix hydrogel to form a microfibrous bundle of dispersed PCL components using elongational and shear stresses within a printing nozzle. By controlling various mixture ratios of alginate and PCL, processing temperature, pneumatic pressure, and nozzle moving speeds, a stable hierarchical structure consisting of PCL microfibers was obtained. To demonstrate the feasibility of hierarchical PCL-based structures coated with type I collagen, we investigated whether the construct affects the alignment and differentiation of myoblasts. The hierarchical fibrous biocomposite constructs presented a higher degree of myogenic activity than the control, which was fabricated using normal printing/collagen coating processes. Furthermore, the hierarchical PCL structures were further elaborated using 10 × simulated body fluid (SBF) resulting in a scaffold consisting of a hydroxyapatite (HA) layer. The hierarchical collagen/PCL/HA structures were evaluated with the aim of obtaining a functional scaffold that could efficiently induce osteogenesis to the human adipose stem cells (hASCs). These in vitro results suggest that the fibrous composite structure can provide enhanced osteogenic activity compared to the normally printed control (collagen/PCL/HA).
논문정보
- Research article
- 2023년 04월 (BRIC 등록일 2023-04-19)
- 국내 연구진
- 바이오·의료융합 > 의료기기
댓글 작성 로그인
팝업 닫기관련 링크
관련분야 연구자보기
소속기관 논문보기
관련분야 논문보기
해당논문 저자보기
