한빛사논문
- 조회536
Gibyuck Byun1,†, Jina Yang6,† and Sang Woo Seo1,2,3,4,5,*
1School of Chemical and Biological Engineering, 1 Gwanak-ro, Gwanak-Gu, Seoul 08826, Korea,
2Interdisciplinary Program in Bioengineering, 1 Gwanak-ro, Gwanak-Gu, Seoul 08826, Korea,
3Institute of Chemical Process, 1 Gwanak-ro, Gwanak-Gu, Seoul 08826, Korea,
4Bio-MAX Institute, 1 Gwanak-ro, Gwanak-Gu, Seoul 08826, Korea,
5Institute of Engineering Research, Seoul National University, 1 Gwanak-ro, Gwanak-Gu, Seoul 08826, Korea and
6Department of Chemical Engineering, Jeju National University, 102, Jejudaehak-ro, Jeju-si, Jeju-do 63243, Korea
*To whom correspondence should be addressed. : Sang Woo Seo
†The authors wish it to be known that, in their opinion, the first two authors should be regarded as Joint First Authors.
Abstract
Precise control of gene expression is essential for flux redistribution in metabolic pathways. Although the CRISPR interference (CRISPRi) system can effectively repress gene expression at the transcriptional level, it has still been difficult to precisely control the level without loss of specificity or an increase in cell toxicity. In this study, we developed a tunable CRISPRi system that performs transcriptional regulation at various levels. We constructed a single-guide RNA (sgRNA) library targeting repeat, tetraloop, and anti-repeat regions to modulate the binding affinity against dCas9. Each screened sgRNA could regulate the gene expression at a certain level between fully-repressing and non-repressing states (>45-fold). These sgRNAs also enabled modular regulation with various target DNA sequences. We applied this system to redistribute the metabolic flux to produce violacein derivatives in a predictable ratio and optimize lycopene production. This system would help accelerate the flux optimization processes in metabolic engineering and synthetic biology.
논문정보
- Research article
- 2023년 03월 (BRIC 등록일 2023-04-05)
- 국내 연구진
- 생명과학 > 미생물학
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