한빛사논문
- 조회444
Seong Goo Kang1†, Yoon Young Choi2†, Sung Jun Mo3, Tae Hyeon Kim4, Jang Ho Ha4, Dong Ki Hong3, Hayera Lee3, Soo Dong Park3, Jae‑Jung Shim3, Jung‑Lyoul Lee3 and Bong Geun Chung2,4*
1Department of Biomedical Engineering, Sogang University, Seoul 04107, Korea.
2Institute of Integrated Biotechnology, Sogang University, Seoul 04107, Korea.
3R&BD Center, hy Co., Ltd., Yongin‑Si, Korea.
4Department of Mechanical Engineering, Sogang University, Seoul 04107, Korea.
†Seong Goo Kang and Yoon Young Choi equally contributed to this work
*Correspondence: Bong Geun Chung
Abstract
Metabolism, is a complex process involving the gut and the liver tissue, is difficult to be reproduced in vitro with conventional single cell culture systems. To tackle this challenge, we developed a gut-liver-axis chip consisting of the gut epithelial cell chamber and three-dimensional (3D) uniform-sized liver spheroid chamber. Two cell culture chamber compartments were separated with a porous membrane to prevent microorganisms from passing through the chamber. When the hepG2 spheroids cultured with microbiota-derived metabolites, we observed the changes in the physiological function of hepG2 spheroids, showing that the albumin and urea secretion activity of liver spheroids was significantly enhanced. Additionally, the functional validation of hepG2 spheroids treated with microbiota-derived exosome was evaluated that the treatment of the microbiota-derived exosome significantly enhanced albumin and urea in hepG2 spheroids in a gut-liver axis chip. Therefore, this gut-liver axis chip could be a potentially powerful co-culture platform to study the interaction of microbiota and host cells.
논문정보
- Research article
- 2023년 01월 (BRIC 등록일 2023-03-30)
- 국내 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
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