한빛사논문
Jinchul Ahn1,2,8, Kyungeun Ohk3,4,8, Jihee Won1,2, Dong-Hee Choi1,2, Yong Hun Jung1,2, Ji Hun Yang2, Yesl Jun5,6, Jin-A Kim1, Seok Chung1,5,7 & Sang-Hoon Lee4,5
1School of Mechanical Engineering, Korea University, Seoul 02841, South Korea.
2Next&Bio Inc., Seoul 02841, South Korea.
3R&D center, Humedix, Co., Ltd., Seongnam 13201, South Korea.
4Department of Bio-convergence Engineering, Korea University, Seoul 02841, South Korea.
5KU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul 02841, South Korea.
6Drug Discovery Platform Research Center, Therapeutics and Biotechnology Division, Korea Research Institute of Chemical Technology, Daejeon 34114, South Korea.
7Center for Brain Technology, Brain Science Institute, Korea Institute of Science and Technology (KIST), Seoul 02792, South Korea.
8These authors contributed equally: Jinchul Ahn, Kyungeun Ohk.
Corresponding authors : Correspondence to Jin-A Kim or Seok Chung.
Abstract
Reconstruction of skin equivalents with physiologically relevant cellular and matrix architecture is indispensable for basic research and industrial applications. As skin-nerve crosstalk is increasingly recognized as a major element of skin physiological pathology, the development of reliable in vitro models to evaluate the selective communication between epidermal keratinocytes and sensory neurons is being demanded. In this study, we present a three-dimensional innervated epidermal keratinocyte layer as a sensory neuron-epidermal keratinocyte co-culture model on a microfluidic chip using the slope-based air-liquid interfacing culture and spatial compartmentalization. Our co-culture model recapitulates a more organized basal-suprabasal stratification, enhanced barrier function, and physiologically relevant anatomical innervation and demonstrated the feasibility of in situ imaging and functional analysis in a cell-type-specific manner, thereby improving the structural and functional limitations of previous coculture models. This system has the potential as an improved surrogate model and platform for biomedical and pharmaceutical research.
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