한빛사논문
- 조회367
Hee Ju Yoo,1 Mi-Young Chung,2 Hyun-Ah Lee,3 Soo-Bin Lee,1 Silvana Grandillo,4 James J. Giovannoni5 and Je Min Lee1,*
1 Department of Horticultural Science, Kyungpook National University, Daegu 41566, Korea
2 Department of Agricultural Education, Sunchon National University, Suncheon 57922, Korea
3 Division of Eco-Friendly Horticulture, Yonam College, Cheonan 31005, Korea
4 CNR-Institute of Bioscience and Bioresources (IBBR), Via Università 133, 80055 Portici, Italy
5 Boyce Thompson Institute and USDA-ARS Robert W. Holley Center, Tower Rd., Cornell University Campus, Ithaca, NY 14853, USA
*Author for correspondence: Je Min Lee
Abstract
Carotenoids and apocarotenoids function as pigments and flavor volatiles in plants that enhance consumer appeal and offer health benefits. Tomato (Solanum lycopersicum.) fruit, especially those of wild species, exhibit a high degree of natural variation in carotenoid and apocarotenoid contents. Using positional cloning and an introgression line (IL) of Solanum habrochaites 'LA1777', IL8A, we identified carotenoid cleavage dioxygenase 4 (CCD4) as the factor responsible for controlling the dark orange fruit color. CCD4b expression in ripe fruit of IL8A plants was ∼8000 times greater than that in the wild type, presumably due to 5' cis-regulatory changes. The ShCCD4b-GFP fusion protein localized in the plastid. Phytoene, ζ-carotene, and neurosporene levels increased in ShCCD4b-overexpressing ripe fruit, whereas trans-lycopene, β-carotene, and lutein levels were reduced, suggestive of feedback regulation in the carotenoid pathway by an unknown apocarotenoid. Solid-phase microextraction-gas chromatography-mass spectrometry analysis showed increased levels of geranylacetone and β-ionone in ShCCD4b-overexpressing ripe fruit coupled with a β-cyclocitral deficiency. In carotenoid-accumulating Escherichia coli strains, ShCCD4b cleaved both ζ-carotene and β-carotene at the C9-C10 (C9'-C10') positions to produce geranylacetone and β-ionone, respectively. Exogenous β-cyclocitral decreased carotenoid synthesis in the ripening fruit of tomato and pepper (Capsicum annuum), suggesting feedback inhibition in the pathway. Our findings will be helpful for enhancing the aesthetic and nutritional value of tomato and for understanding the complex regulatory mechanisms of carotenoid and apocarotenoid biogenesis.
논문정보
- Research article
- 2023년 01월 (BRIC 등록일 2023-03-13)
- 국내(교신)+국외 연구진
- 생명과학 > 식량작물 및 원예작물
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