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조회318

인천대학교

Carbohydr. Polym., 2023 Mar 15;304:120490 | https://doi.org/10.1016/j.carbpol.2022.120490 Accurate detection of enzymatic degradation processes of gelatin–alginate microcapsule by 1H NMR spectroscopy: Probing biodegradation mechanism and kinetics

Uyen Thi Do ^a,1, Jiwon Kim^a,1, Quy Son Luu ^a, Quynh Thi Nguyen ^b, Taeho Jang^b, Yeeun Park ^c, Hwicheol Shin ^d, Nicholas Whiting ^e, Dong-Ku Kang ^d, Jas-Sung Kwon ^f,g, Youngbok Lee ^a,b,c

^aDepartment of Bionano Technology, Hanyang University, Ansan 15588, South Korea
^bDepartment of Applied Chemistry, Center for Bionano Intelligence Education and Research, Hanyang University, Ansan 15588, South Korea
^cDepartment of Chemical and Molecular Engineering, Hanyang University, Ansan 15588, South Korea
^dDepartment of Chemistry, Incheon National University, Incheon 22012, South Korea
^eDepartment of Physics & Astronomy and Department of Biological & Biomedical Sciences, Rowan University, Glassboro, NJ 08028, USA
^fDepartment of Mechanical Engineering, Incheon National University, Incheon 22012, South Korea
^gConvergence Research Center for Insect Vectors(CRCIV), Incheon National University, Incheon 22012, South Korea

¹These authors contributed equally to this work.
Corresponding authors: Dong-Ku Kang, Jas-Sung Kwon, Youngbok Lee

Abstract

With an increase in the severity of environmental pollution caused by microbeads, the development of biodegradable microcapsules that can be applied in diverse fields has attracted significant attention. The degradation processes are directly related to biodegradable microcapsule creation with high stability and persistence. In this study, biodegradable microcapsules are synthesized via a complex coacervation approach using gelatin and alginate as the capsule main wall materials; additionally, enzyme-induced decomposition mechanisms are proposed by observing spectral changes in proton nuclear magnetic resonance (¹H NMR) analyses. Additional analytical techniques confirm the chemical structure, morphology, and size distribution of the synthesized capsules; these uniform spherical microcapsules are 20-30 μm in size and possess a smooth surface. In addition to characterization, the microcapsules were exposed to targeted enzymes to investigate enzymatic effects using short-term and long-term degradation kinetics. Close inspection reveals that determination of the degradation rate constant of the major components in the capsule is feasible, and suggests two types of 4-stage degradation mechanisms that are enzyme-specific. These investigations demonstrate that capsule degradation can be explored in detail using ¹H NMR spectroscopy to provide a viable strategy for monitoring degradation properties in the development of new biodegradable polymers.

논문정보

형식Research article
게재일2023년 03월 (BRIC 등록일 2023-03-17)
연구진국내(교신)+국외 연구진
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