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Nat. Commun., 2023 Feb 24;14(1):1055 | https://doi.org/10.1038/s41467-023-36691-x Single-cell transcriptome profiling of the stepwise progression of head and neck cancer

Ji-Hye Choi^1,2,6, Bok-Soon Lee^3,6, Jeon Yeob Jang^2,3,6, Yun Sang Lee³, Hyo Jeong Kim^3,4, Jin Roh⁵, Yoo Seob Shin³, Hyun Goo Woo^1,2 & Chul-Ho Kim^3,5

¹Departent of Physiology, Ajou University School of Medicine, Suwon, Republic of Korea.
²Department of Biomedical Science, Graduate School, Ajou University, Suwon, Republic of Korea.
³Department of Otolaryngology, Ajou University School of Medicine, Suwon, Republic of Korea.
⁴Department of Molecular Science and Technology, Ajou University, Suwon, Republic of Korea.
⁵Department of Pathology, Ajou University School of Medicine, Suwon, Republic of Korea.
⁶These authors contributed equally: Ji-Hye Choi, Bok-Soon Lee, Jeon Yeob Jang.

Corresponding authors : Correspondence to Hyun Goo Woo or Chul-Ho Kim.

Abstract

Head and neck squamous cell carcinoma (HNSCC) undergoes stepwise progression from normal tissues to precancerous leukoplakia, primary HNSCC, and metastasized tumors. To delineate the heterogeneity of tumor cells and their interactions during the progression of HNSCC, we employ single-cell RNA-seq profiling for normal to metastasized tumors. We can identify the carcinoma in situ cells in leukoplakia lesions that are not detected by pathological examination. In addition, we identify the cell type subsets of the Galectin 7B (LGALS7B)-expressing malignant cells and CXCL8-expressing fibroblasts, demonstrating that their abundance in tumor tissue is associated with unfavorable prognostic outcomes. We also demonstrate the interdependent ligand-receptor interaction of COL1A1 and CD44 between fibroblasts and malignant cells, facilitating HNSCC progression. Furthermore, we report that the regulatory T cells in leukoplakia and HNSCC tissues express LAIR2, providing a favorable environment for tumor growth. Taken together, our results update the pathobiological insights into cell-cell interactions during the stepwise progression of HNSCCs.

논문정보

형식Research article
게재일2023년 02월 (BRIC 등록일 2023-03-06)
연구진국내 연구진
분야 의약학 > 종양의학

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