한빛사논문
- 조회349
한국과학기술연구원
Bong Kyun Kim 1,2,3, Sang-A Lee 3, Minju Park 4, Eui Ju Jeon 3, Mi Jung Kim 3, Jung Min Kim 3, Heesuk Kim 4,5, Seungwon Jung 6,7,3, Sang Kyung Kim 6,8,3
1Division of Bio-Medical Science & Technology, KIST School, Korea University of Science and Technology (UST), Daejeon 34113, Korea.
2BioActs Co., Ltd., Incheon 21666, Korea.
3Center for Augmented Safety Systems with Intelligence, Sensing and Tracking (ASSIST), KIST, Seoul 02792, Korea.
4Soft Hybrid Materials Research Center, KIST, Seoul 02792, Korea.
5Division of Energy and Environmental Technology, KIST School, UST, Daejeon 34113, Korea.
6Center for Advanced Biomolecular Recognition, KIST, Seoul 02792, Korea.
7Department of HY-KIST Bio-convergence, Hanyang University, Seoul 04763, Korea.
8KHU-KIST Department of Converging Science and Technology, Kyung Hee University, Seoul 02447, Korea.
Corresponding Authors : Seungwon Jung, Sang Kyung Kim
Abstract
As the turnaround time of diagnosis becomes important, there is an increasing demand for rapid, point-of-care testing (POCT) based on polymerase chain reaction (PCR), the most reliable diagnostic tool. Although optical components in real-time PCR (qPCR) have quickly become compact and economical, conventional PCR instruments still require bulky thermal systems, making it difficult to meet emerging needs. Photonic PCR, which utilizes photothermal nanomaterials as heating elements, is a promising platform for POCT as it reduces power consumption and process time. Here, we develop a photonic qPCR platform using hydrogel microparticles. Microparticles consisting of hydrogel matrixes containing photothermal nanomaterials and primers are dubbed photothermal primer-immobilized networks (pPINs). Reduced graphene oxide is selected as the most suitable photothermal nanomaterial to generate heat in pPIN due to its superior light-to-heat conversion efficiency. The photothermal reaction volume of 100 nL (predefined by the pPIN dimensions) provides fast heating and cooling rates of 22.0 ± 3.0 and 23.5 ± 2.6 °C s-1, respectively, enabling ultrafast qPCR within 5 min only with optical components. The microparticle-based photonic qPCR facilitates multiplex assays by loading multiple encoded pPIN microparticles in a single reaction. As a proof of concept, four-plex pPIN qPCR for bacterial discrimination are successfully demonstrated.
논문정보
- Research article
- 2022년 12월 (BRIC 등록일 2023-02-27)
- 국내 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
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