한빛사논문
- 조회409
Narendra K Singh1,2,3, Jae Yun Kim4, Jae Yeon Lee5, Hyungseok Lee1,6,7, Ge Gao1,8, Jinah Jang1,4,9,10, Yong Kyun Kim11,12,13 and Dong-Woo Cho14,1,10
1 Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Republic of Korea
2 Division of Biomaterials and Biomechanics, School of Dentistry, Oregon Health and Science University (OHSU), Portland, OR 97201, United States of America
3 Cancer Early Detection Advanced Research Center (CEDAR), OHSU-Knight Cancer Institute, Portland, OR 97201, United States of America
4 School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH), Pohang, Republic of Korea
5 Department of Companion Animal Health, Daegu Haany University, Gyeongsan, Republic of Korea
6 Department of Mechanical and Biomedical Engineering, Kangwon National University (KNU), Chuncheon, Republic of Korea
7 Interdisciplinary Program in Biohealth-Machinery Convergence Engineering, Kangwon National University (KNU), Chuncheon, Republic of Korea
8 Institute of Engineering Medicine, Beijing Institute of Technology, Beijing, People's Republic of China
9 Department of Convergence IT Engineering, Pohang University of Science and Technology (POSTECH), Pohang, Republic of Korea
10 Institute for Convergence Research and Education in Advanced Technology, Yonsei University, Seoul, Republic of Korea
11 Cell Death Disease Research Center, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea
12 Department of Internal Medicine, College of Medicine, The Catholic University of Korea, St. Vincent's Hospital, Suwon, Republic of Korea
13 POSTECH-Catholic Biomedical Engineering Institute, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea
14 Author to whom any correspondence should be addressed.
Abstract
Much effort has been expended in emulating the kidney's glomerular unit because of its limitless potential in the field of drug screening and nephrotoxicity testing in clinics. Herein, we fabricate a functional bilayer glomerular microvessel-on-a-chip that recapitulates the specific arrangement of the glomerular endothelial cell, podocyte layers, and the intervening glomerular basement membrane (GBM) in a single step. Our perfusable chip allows for the co-culture of monolayer glomerular endothelium and podocyte epithelium, which display mature functional markers of glomerular cells, and their proper interactions produce GBM proteins, which are the major components of the GBM in vivo. Furthermore, we test the selective permeability capacity, a representative hallmark function of the glomerular filtration barrier. Lastly, we evaluate the response of our glomerular model to Adriamycin- and hyperglycemia-induced injury to evaluate its applicability for drug screening and glomerular disease modeling.
논문정보
- Research article
- 2023년 01월 (BRIC 등록일 2023-02-13)
- 국내(교신)+국외 연구진
- 바이오·의료융합 > 의료기기
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