한빛사논문
- 조회331
Jinhwan Kim a,1, Cheonjung Kim a,b,1, Jeong Soo Park a, Na Eun Lee a,c, Seungmin Lee a,d, Sung-Yeon Cho e,f, Chulmin Park e, Dae Sung Yoon d, Yong Kyoung Yoo b, Jeong Hoon Lee a
aDepartment of Electrical Engineering, Kwangwoon University, Seoul, 01897, Republic of Korea
bDepartment of Electronic Engineering, Catholic Kwandong University, Gangneung-si, Gangwon-do, 25601, Republic of Korea
cDepartment of Biotechnology, College of Life Sciences and Biotechnology, Korea University, Seoul, 02841, Republic of Korea
dSchool of Biomedical Engineering, Korea University, Seoul, 02841, Republic of Korea
eVaccine Bio Research Institute, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea
fDivision of Infectious Diseases, Department of Internal Medicine, Seoul St. Mary's Hospital, Catholic Hematology Hospital, College of Medicine, The Catholic University of Korea, Seoul 06591, Republic of Korea
1These authors contributed equally.
Corresponding authors: Dae Sung Yoon, Yong Kyoung Yoo, Jeong Hoon Lee
Abstract
A simple, affordable point of care test (POCT) is necessary for on-site detection of coronavirus disease 2019 (COVID-19). The lateral flow assay (LFA) has great potential for use in POCT mainly because of factors such as low time consumption, low cost, and ease of use. However, it lacks sensitivity and limits of detection (LOD), which are essential for early diagnostics. In this study, we proposed a non-powered preconcentrator (NPP) based on nanoelectrokinetics for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Antigen (Ag) lateral flow assay. The non-powered preconcentrator is composed of glass fiber-based composite paper and ion permselective material, and it can be simply operated by force balancing gravitational, capillary, and depletion-induced forces. The proposed approach helps enrich the SARS-CoV-2 viral nucleocapsid (N) proteins based on a 10-min operation, and it improved the LOD by up to 10-fold. The corresponding virus enrichment, which was evaluated using the reverse-transcriptase polymerase chain reaction (RT-PCR), revealed an improvement in ΔCt values > 3. We successfully demonstrated the enhancement of the NPP-assisted LFA, we extended to applying it to clinical samples. Further, we demonstrated an affordable, easy-to-implement form of LFA by simply designing NPP directly on the LFA buffer tube.
논문정보
- Research article
- 2023년 02월 (BRIC 등록일 2023-02-09)
- 국내 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
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