한빛사논문
- 조회434
Seul Gi Kim, Jinsoo Song, Benny Ryplida, Hyeong Jun Jo, Gun-Jae Jeong, Innie Y. Kang, Jay Milan Patel, Eun-Jung Jin,* Young C. Jang,* and Sung Young Park*
S. G. Kim, S. Y. Park
Department of Chemical and Biological Engineering, Korea National University of Transportation, Chungju 27469, Republic of Korea
S. G. Kim, B. Ryplida, S. Y. Park
Department of Green Bio Engineering, Korea National University of Transportation, Chungju 27469, Republic of Korea
J. Song, E.-J. Jin
Department of Biological Sciences, College of Natural Sciences, Wonkwang University, Chunbuk 54538, Iksan, Republic of Korea
H. J. Jo, S. Y. Park
Department of IT and Energy Convergence (BK21 FOUR), Korea National University of Transportation, Chungju 27469, Republic of Korea
G.-J. Jeong, I. Y. Kang, J. M. Patel, Y. C. Jang
Department of Orthopaedics, Emory Musculoskeletal Institute, Emory University School of Medicine, Atlanta, GA 30329, USA
Y. C. Jang
Wallace H. Coulter Department of Biomedical Engineering/Petit H. Parker, Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, GA 30332, USA
CORRESPONDING AUTHORS: Eun-Jung Jin, Young C. Jang, Sung Young Park
Abstract
In this study, a reactive oxygen species (ROS)-responsive hydrogel sensor (PD/MnO2 hydrogel) is developed that can efficiently detect senescent cells. Using immature murine articular chondrocytes with serial passages, the sensor can identify small interfering RNA (siRNA) knockdown of peroxisome proliferator-activated receptor-alpha (PPARα) based on the concentration of ROS in cells, simultaneously maintaining its balance via scavenging activity to prevent cartilage degradation in osteoarthritis (OA). The hydrogel sensor exhibits a change in electronic properties, with a distinct resistance from 201.9 kΩ for P0 to 362.9 kΩ for P3, and fluorescence off/on performance with an increase in passaging time. In vitro investigation using PPARα-specific siRNA reveals a correlation between pressure sensitivity and senescent activity, wherein an elevation in observed signal occurred (41.5%). In vivo analysis reveals significant decrease in degradation of the cartilage of both young, 3 months old aged, 18 months old, and PPARα−/− mice compared to PPARα+/+ mice based on safranin O stains. The expression level of interleukin-1β is reduced in the cartilage of aged PPARα−/− mice after implantation with hydrogel, indicating the potential of PD/MnO2 hydrogel as a therapeutic modality against OA.
논문정보
- Research article
- 2023년 01월 (BRIC 등록일 2023-02-08)
- 국내(교신)+국외 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
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