한빛사논문
- 조회260
한국과학기술연구원(KIST)
Seung‑Hoon Um1,2†, Youngmin Seo1,3†, Hyunseon Seo1,4, Kyungwoo Lee1, Sun Hwa Park2, Jung Ho Jeon5,6, Jung Yeon Lim5, Myoung‑Ryul Ok1, Yu‑Chan Kim1,7, Hyunjung Kim8, Cheol‑Hong Cheon9, Hyung‑Seop Han1, James R. Edwards10, Sung Won Kim5,6* and Hojeong Jeon1,7,11*
1Biomaterials Research Center, Biomedical Research Division, Korea Institute of Science and Technology (KIST), 02792 Seoul, Republic of Korea.
2Laboratory for Biomaterials and Bioengineering, Department of Min‑Met‑Materials Engi‑neering, Research Center of CHU de Quebec, Division of Regenerative Medi‑cine, Canada Research Chair I in Biomaterials and Bioengineering for the Inno‑vation in Surgery, Laval University, G1V 0A6 Quebec City, Quebec, Canada.
3R&D Institute, OID Ltd, Seoul 06286, Republic of Korea.
4School of Medicine, Sungkyunkwan University, Suwon 16419, Republic of Korea.
5Department of Otolaryngology‑Head and Neck Surgery, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
6Department of Biomedicine & Health Sciences, Department of Otolaryngology‑Head and Neck Surgery, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
7Division of Bio‑Medical Science and Technology, KIST School, Korea University of Science and Technology, Seoul 02792, Republic of Korea.
8Division of Nurs‑ing, Research Institute of Nursing Science, Hallym University, Chuncheon 24252, Republic of Korea.
9Department of Chemistry, Korea University, Seoul 02841, Republic of Korea.
10Nufeld Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences (NDORMS), Botnar Research Centre, University of Oxford, Oxford OX3 7LD, UK.
11KU‑KIST Graduate School of Converging Sci‑ence and Technology, Korea University, Seoul 02841, Republic of Korea.
†Seung-Hoon Um and Youngmin Seo contributed equally to this work.
*Correspondence: Sung Won Kim, Hojeong Jeon
Abstract
Background: Cells in the human body experience different growth environments and conditions, such as compressive pressure and oxygen concentrations, depending on the type and location of the tissue. Thus, a culture device that emulates the environment inside the body is required to study cells outside the body.
Methods: A blanket-type cell culture device (Direct Contact Pressing: DCP) was fabricated with an alginate-based hydrogel. Changes in cell morphology due to DCP pressure were observed using a phase contrast microscope. The changes in the oxygen permeability and pressure according to the hydrogel concentration of DCP were analyzed. To compare the effects of DCP with normal or artificial hypoxic cultures, cells were divided based on the culture technique: normal culture, DCP culture device, and artificial hypoxic environment. Changes in phenotype, genes, and glycosaminoglycan amounts according to each environment were evaluated. Based on this, the mechanism of each culture environment on the intrinsic properties of conserving chondrocytes was suggested.
Results: Chondrocytes live under pressure from the surrounding collagen tissue and experience a hypoxic environment because collagen inhibits oxygen permeability. By culturing the chondrocytes in a DCP environment, the capability of DCP to produce a low-oxygen and physical pressure environment was verified. When human primary chondrocytes, which require pressure and a low-oxygen environment during culture to maintain their innate properties, were cultured using the hydrogel blanket, the original shapes and properties of the chondrocytes were maintained. The intrinsic properties could be recovered even in aged cells that had lost their original cell properties.
Conclusions: A DCP culture method using a biomimetic hydrogel blanket provides cells with an adjustable physical pressure and a low-oxygen environment. Through this technique, we could maintain the original cellular phenotypes and intrinsic properties of human primary chondrocytes. The results of this study can be applied to other cells that require special pressure and oxygen concentration control to maintain their intrinsic properties. Additionally, this technique has the potential to be applied to the re-differentiation of cells that have lost their original properties.
논문정보
- Research article
- 2022년 12월 (BRIC 등록일 2023-03-06)
- 국내(교신)+국외 연구진
- 바이오·의료융합 > 바이오소재
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