한빛사논문
- 조회347
Gwang-Bum Im1, Yu-Jin Kim1, Tae Il Lee2, Suk Ho Bhang1
1School of Chemical Engineering, Sungkyunkwan University, Suwon, Republic of Korea
2Department of Materials Science and Engineering, Gachon University, Seongnam,Republic of Korea
Gwang-Bum Im and Yu-Jin Kim contributed equally to this work.
Correspondence: Suk Ho Bhang, Tae Il Lee
Present address: Gwang-Bum Im, Department of Cardiac Surgery, Boston Children's Hospital and Harvard Medical School, Boston, Massachusetts, USA.
Abstract
Conventional 3D cell culture methods require a comprehensive complement in labor-intensive and time-consuming processes along with in vivo circumstantial mimicking. Here, we describe a subaqueous free-standing 3D cell culture (FS) device that can induce the omnidirectional environment and generate ultrafast human adipose-derived stem cells (hADSCs) that efficiently aggregate with compaction using acoustic pressure. The cell culture conditions were optimized using the FS device and identified the underlying molecular mechanisms. Unique phenomena in cell aggregation have led to extraordinary cellular behavior that can upregulate cell compaction, mechanosensitive immune control, and therapeutic angiogenesis. Therefore, we designated the resulting cell aggregates as “pressuroid.” Notably, external acoustic stimulation produced by the FS device affected the pressuroids. Furthermore, the pressuroids exhibited upregulation in mechanosensitive genes and proteins, PIEZO1/2. CyclinD1 and PCNA, which are strongly associated with cell adhesion and proliferation, were elevated by PIEZO1/2. In addition, we found that pressuroids significantly increase angiogenic paracrine factor secretion, promote cell adhesion molecule expression, and enhance M2 immune modulation of Thp1 cells. Altogether, we have concluded that our pressuroid would suggest a more effective therapy method for future cell therapy than the conventional one.
논문정보
- Research article
- 2022년 10월 (BRIC 등록일 2023-01-30)
- 국내 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
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