장영우 (Stanford University, 현 가천대학교 의과대학)

한빛사논문

조회291

Stanford University, 현 가천대학교 의과대학

CV File 252 kb

ACS Nano, 2018 Mar 27;12(3):2253-2266 | https://doi.org/10.1021/acsnano.7b06212 Effect of Cell Sex on Uptake of Nanoparticles: The Overlooked Factor at the Nanobio Interface

Vahid Serpooshan^,†,‡,⬡ Sara Sheibani,^§,⬡ Pooja Pushparaj^,∥,⬡ Michal Wojcik,^⊥,⬡ Albert Y. Jang,^#,⬡ Michelle R. Santoso,^# Joyce H. Jang,^¶ Haina Huang,^⊥ Reihaneh Safavi-Sohi,^□ Niloofar Haghjoo,^△ Hossein Nejadnik,^▽ Haniyeh Aghaverdi,^▲ Hojatollah Vali,^§ Joseph Matthew Kinsella,^*,∥ John Presley,*,§ Ke Xu,*,⊥,▼ Phillip Chung-Ming Yang,*,# and Morteza Mahmoudi*,#,▲

^†Department of Biomedical Engineering, Georgia Institute of Technology & Emory University School of Medicine, Atlanta, Georgia 30322, United States
^‡Department of Pediatrics, Emory University School of Medicine, Atlanta, Georgia 30322, United States
^§Department of Anatomy and Cell Biology and Facility for Electron Microscopy Research, McGill University, Montreal, Quebec H3A 0C3, Canada
^∥Department of Bioengineering, McGill University, Montreal, Quebec H3A 0C3, Canada
^⊥Department of Chemistry, University of California, Berkeley, California 94720, United States
^#Division of Cardiovascular Medicine, Stanford University, Stanford, California 94305, United States
^¶Meakins Christie Laboratories, McGill University Health Centre and McGill University, Montreal, Quebec H4A 3J1, Canada
^□Department of Phytochemistry, Medicinal Plants and Drugs Research Institute, Shahid Beheshti University, Tehran 1983963113, Iran
^△Institute of Biochemistry and Biophysics, University of Tehran, Tehran 14174, Iran
^▽Department of Radiology and Molecular Imaging Program at Stanford (MIPS), Stanford School of Medicine, Stanford,
California 94305, United States
^▲Department of Anesthesiology, Brigham & Women’s Hospital, Harvard Medical School, Boston, Massachusetts 02115, United States
^▼Division of Molecular Biophysics and Integrated Bioimaging, Lawrence Berkeley National Laboratory, Berkeley, California 94720,
United States

^⬡V. Serpooshan, S. Sheibani, P. Pushparaj, M. Wojcik, and A. Y. Jang contributed equally to this work.
*Corresponding Authors : Joseph Matthew Kinsella, John Presley, Ke Xu, Morteza Mahmoudi

Abstract

Cellular uptake of nanoparticles (NPs) depends on the nature of the nanobio system including the solid nanocomponents ( e. g., physicochemical properties of NPs), nanobio interfaces ( e. g., protein corona composition), and the cellular characteristics ( e. g., cell type). In this study, we document the role of sex in cellular uptake of NPs as an "overlooked" factor in nanobio interface investigations. We demonstrate that cell sex leads to differences in NP uptake between male and female human amniotic stem cells (hAMSCs), with greater uptake by female cells. hAMSCs are one of the earliest sources of somatic stem cells. The experiments were replicated with primary fibroblasts isolated from the salivary gland of adult male and female donors of similar ages, and again the extent of NP uptake was altered by cell sex. However, in contrast to hAMSCs, uptake was greater in male cells. We also found out that female versus male amniotic stem cells exhibited different responses to reprogramming into induced pluripotent stem cells (iPSCs) by the Yamanaka factors. Thus, future studies should consider the effect of sex on the nanobio interactions to optimize clinical translation of NPs and iPSC biology and to help researchers to better design and produce safe and efficient therapeutic sex-specific NPs.

논문정보

형식Research article
게재일2018년 03월 (BRIC 등록일 2023-01-26)
연구진국외 연구진
분야 의약학 > 응용의학

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