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조회275

원재혁Jaehyuk Won

중앙대학교

Nat. Commun., 2022 Nov 7;13(1):6506 | https://doi.org/10.1038/s41467-022-34190-z Circuit-guided population acclimation of a synthetic microbial consortium for improved biochemical production

Chae Won Kang^1,6, Hyun Gyu Lim^1,6, Jaehyuk Won^2,3,6, Sanghak Cha¹, Giyoung Shin⁴, Jae-Seong Yang⁵, Jaeyoung Sung^2,3 & Gyoo Yeol Jung^1,4

¹Department of Chemical Engineering, Pohang University of Science and Technology, 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk 37673, Korea.
²Creative Research Initiative Center for Chemical Dynamics in Living Cells, Chung-Ang University, 84 Heukseok-Ro, Dongjak-gu, Seoul 06974, Republic of Korea.
³Department of Chemistry, Chung-Ang University, 84 Heukseok-Ro, Dongjak-gu, Seoul 06974, Republic of Korea.
⁴School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology, 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk 37673, Korea.
⁵Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Campus UAB, Bellaterra, Barcelona 08193, Spain.
⁶These authors contributed equally: Chae Won Kang, Hyun Gyu Lim, Jaehyuk Won.

Corresponding author : Correspondence to Gyoo Yeol Jung.

Abstract

Microbial consortia have been considered potential platforms for bioprocessing applications. However, the complexity in process control owing to the use of multiple strains necessitates the use of an efficient population control strategy. Herein, we report circuit-guided synthetic acclimation as a strategy to improve biochemical production by a microbial consortium. We designed a consortium comprising alginate-utilizing Vibrio sp. dhg and 3-hydroxypropionic acid (3-HP)-producing Escherichia coli strains for the direct conversion of alginate to 3-HP. We introduced a genetic circuit, named "Population guider", in the E. coli strain, which degrades ampicillin only when 3-HP is produced. In the presence of ampicillin as a selection pressure, the consortium was successfully acclimated for increased 3-HP production by 4.3-fold compared to that by a simple co-culturing consortium during a 48-h fermentation. We believe this concept is a useful strategy for the development of robust consortium-based bioprocesses.

논문정보

형식Research article
게재일2022년 11월 (BRIC 등록일 2023-01-19)
연구진국내(교신)+국외 연구진
분야 생명과학 > 생물공학

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