한빛사논문
- 조회201
성균관대학교
Mookwang Kwon1, Ju Hyun Lee2, Youngik Yoon2, Samuel J Pleasure3, and Keejung Yoon*1,2
1Department of Integrative Biotechnology, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon, South Korea
2Department of Biopharmaceutical Convergence, Sungkyunkwan University, Suwon, South Korea
3Department of Neurology, University of California San Francisco, San Francisco, CA, USA
*Corresponding author: Keejung Yoon
Abstract
Growing evidence suggests that the corticotropin-releasing hormone (CRH) signaling pathway, mainly known as a critical initiator of humoral stress responses, has a role in normal neuronal physiology. However, despite the evidence of CRH receptor (CRHR) expression in the embryonic ventricular zone, the exact functions of CRH signaling in embryonic brain development have not yet been fully determined. In this study, we show that CRHR1 is required for the maintenance of neural stem cell properties, as assessed by in vitro neurosphere assays and cell distribution in the embryonic cortical layers following in utero electroporation. Identifying the underlying molecular mechanisms of CRHR1 action, we find that CRHR1 functions are accomplished through the increasing expression of the master transcription factor REST. Furthermore, luciferase reporter and chromatin immunoprecipitation assays reveal that CRHR1-induced CREB activity is responsible for increased REST expression at the transcriptional level. Taken together, these findings indicate that the CRHR1/CREB/REST signaling cascade plays an important role downstream of CRH in the regulation of neural stem cells during embryonic brain development.
논문정보
- Research article
- 2022년 11월 (BRIC 등록일 2023-01-26)
- 국내(교신)+국외 연구진
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