한빛사논문
- 조회376
Yoon Jeong1,2, Joseph Irudayaraj1,2,3
1Department of Bioengineering, University of Illinois at Urbana‐Champaign, Urbana, Illinois, USA
2Cancer Center at Illinois, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA
3Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois, USA
Corresponding author: Joseph Irudayaraj
Abstract
To sequester prokaryotic cells in a biofilm-like niche, the creation of a pertinent and reliable microenvironment that reflects the heterogeneous nature of biological systems is vital for sustenance. Design of a microenvironment that is conducive for growth and survival of organisms, should account for factors such as mass transport, porosity, stability, elasticity, size, functionality, and biochemical characteristics of the organisms in the confined architecture. In this work we present an artificial long-term confinement model fabricated by natural alginate hydrogels that are structurally stable and can host organisms for over 10 days in physiologically relevant conditions. A unique feature of the confinement platform is the development of stratified habitats wherein bacterial cells can be entrapped in the core as well as in the shell layers, wherein the thickness and the number of shell layers are tunable at fabrication. We show that the hydrogel microenvironment in the beads can host complex subpopulations of organisms similar to that in a biofilm. Dynamic interaction of bacterial colonies encapsulated in different beads or within the core and stratified layers of single beads was demonstrated to show intra- species communication. Inter- species communication between probiotic bacteria and human colorectal carcinoma cells was also demonstrated to highlight a possible bidirectional communication between the organisms in the beads and the environment.
논문정보
- Research article
- 2023년 01월 (BRIC 등록일 2023-01-11)
- 국외 연구진
- 생명과학 > 생물공학
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