Iris Baffour Ansah (한국재료연구원(KIMS)) | 한빛사논문 > 한빛사

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Iris Baffour AnsahIris Baffour Ansah

한국재료연구원(KIMS)

Biosens. Bioelectron., Volume 220, 15 January 2023, 114930 | https://doi.org/10.1016/j.bios.2022.114930 In-situ fabrication of 3D interior hotspots templated with a protein@Au core-shell structure for label-free and on-site SERS detection of viral diseases

Iris Baffour Ansah^a,b,1, Soo Hyun Lee^a,1, Jun-Yeong Yang^a, ChaeWon Mun^a, Sunghoon Jung^a, Ho Sang Jung^a, Min-Young Lee^a, Taejoon Kang^c,d, Seunghun Lee^a, Dong-Ho Kim^a,b, Sung-Gyu Park^a

^aNano-Bio Convergence Department, Korea Institute of Materials Science (KIMS), Changwon, Gyeongnam, 51508, Republic of Korea
^bAdvanced Materials Engineering Division, University of Science and Technology (UST), Daejeon, 34113, Republic of Korea
^cBionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, 34141, Republic of Korea
^dSchool of Pharmacy, Sungkyunkwan University, Suwon, 16419, Republic of Korea

¹These authors contributed equally to this work.
Corresponding author; Sung-Gyu Park

Abstract

Nanoscale plasmonic hotspots play a critical role in the enhancement of molecular Raman signals, enabling the sensitive and reliable trace analysis of biomedical molecules via surface-enhanced Raman spectroscopy (SERS). However, effective and label-free SERS diagnoses in practical fields remain challenging because of clinical samples' random adsorption and size mismatch with the nanoscale hotspots. Herein, we suggest a novel SERS strategy for interior hotspots templated with protein@Au core-shell nanostructures prepared via electrochemical one-pot Au deposition. The cytochrome c and lysates of SARS-CoV-2 (SLs) embedded in the interior hotspots were successfully functionalized to confine the electric fields and generate their optical fingerprint signals, respectively. Highly linear quantitative sensitivity was observed with the limit-of-detection value of 10^-1 PFU/mL. The feasibility of detecting the targets in a bodily fluidic environment was also confirmed using the proposed templates with SLs in human saliva and nasopharyngeal swabs. These interior hotspots templated with the target analytes are highly desirable for early and on-site SERS diagnoses of infectious diseases without any labeling processes.

논문정보

형식Research article
게재일2023년 01월 (BRIC 등록일 2023-01-26)
연구진국내 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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