한빛사논문
- 조회333
Saeed Ur Rahmana,1, Woo-Jin Kima,1, Shin Hye Chungb, Kyung Mi Wooa,c
aDepartment of Molecular Genetics, Dental Research Institute, School of Dentistry, Seoul National University, Seoul, 08826, Republic of Korea
bDepartment of Dental Biomaterials Science, Dental Research Institute, School of Dentistry, Seoul National University, Seoul, 03080, Republic of Korea
cDepartment of Pharmacology & Dental Therapeutics, School of Dentistry, Seoul National University, Seoul, 08826, Republic of Korea
1These authors contributed equally.
Corresponding author: Kyung Mi Woo
Abstract
Cell differentiation with the proper three-dimensional (3-D) structure is critical for cells to carry out their cellular functions in tissues. Odontoblasts derived from neural crest cells are elongated and polarized with the cell process, which is decisive for one directional tubular dentin formation. Here, we report that the fibrous topography of scaffolds directs odontoblast-lineage cells to differentiate to have the 3-D structure of odontoblasts through an altered responsiveness to Wnt family member 5A (Wnt5a). In a pulp-exposure animal model, the scaffolds with the nanofibrous topography supported the regeneration of tubular dentin with odontoblast processes. In cultures of pre-odontoblast cells, the nanofibrous topography heightened the cells on the z-axis. The cells on nanofibrous substrate (FIBER) formed stress fiber cytoskeletons on a conventional tissue culture plate (TCP). Differential activation of Cell division control protein 42 (Cdc42) on FIBER and Ras homolog family member A (RhoA) on TCP led to these differences. The signal from Wnt5a-Cdc42 in the cells on FIBER mediated the phosphorylation of JNK and the polarity growth signaling. Taken together, the nanofibrous topography of the scaffolds led to the 3-D structural differentiation of odontoblasts in vitro and in vivo, implying its application for dentin regeneration. Furthermore, the results on the altered activation of Cdc42 by Wnt5a on FIBER provide evidence that the topography of the scaffolds can cause a distinctive cell responsiveness to their micro-environments.
논문정보
- Research article
- 2022년 12월 (BRIC 등록일 2023-01-13)
- 국내 연구진
- 의약학 > 치의학
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