박성욱 (한국과학기술연구원(KIST), UST) | 한빛사논문 > 한빛사

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한국과학기술연구원(KIST), UST

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Chem. Eng. J., Volume 456, 15 Jan 2023, 141079 | https://doi.org/10.1016/j.cej.2022.141079 Modular conductive MOF-gated field-effect biosensor for sensitive discrimination on the small molecular scale

Changjoon Keum^a,1, Sungwook Park^a,1, Hyunro Kim^a,b,1, Hojun Kim^a, Kwan Hyi Lee^a,b, Youngdo Jeong^a,c

^aCenter for Advanced Biomolecular Recognition, Biomedical Research Division, Korea Institute of Science and Technology (KIST), Seoul 02792, Republic of Korea
^bKU-KIST Graduate School of Converging Science and Technology, Korea University, Seoul 02841, Republic of Korea
^cDepartment of HY-KIST Bio-convergence, Hanyang University, Seoul 04763, Republic of Korea

¹These authors contributed equally.
Corresponding authors : Kwan Hyi Lee, Youngdo Jeong

Abstract

Field-effect transistors (FETs) are a promising transducer for biosensors owing to their drastic signal amplification, showing a high sensitivity enough to detect ultra-low concentrations of small-molecular biomarkers in biofluids. However, the discrimination of small molecules from their structural analogues in biofluids using a FET is challenging; because of the 1) Debye screening in high-ionic-strength conditions, 2) few or no charges of the analytes, and 3) lack of target-specific receptors for small molecules. To overcome these limitations, we report a modular conductive MOF (c-MOF)-gated FET biosensor array that discriminates small-molecular neurotransmitters in biofluids. Adsorption and oxidation of analytes inside the pore of catalytic c-MOF films allow the sensitive detection of small molecules close to the clinically relevant concentration, regardless of the ionic strength of the media. The hierarchical screening ability of c-MOF attributed to their tunable pore size, surface charge, and host–guest interactions induces differentiated adsorption of small molecules. The cross-reaction intended by c-MOF design of our biosensor could provide discriminatory information of each neurotransmitter corresponding to the differences of one functional group in the molecule. Using our system, we also demonstrate discrimination capability under the interference of biologically relevant substances and artificial cerebrospinal fluid.

논문정보

형식Research article
게재일2023년 01월 (BRIC 등록일 2023-01-09)
연구진국내 연구진
분야 바이오·의료융합 > 바이오센싱 및 나노바이오물질

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