한빛사논문
연세대학교
Young-Bo Sima, Jisu Yanga, Saint Moon Kima, Hwan-Hong Jooa, Ju-Hyeong Jungb, Do-Hyung Kimc, Sang-Hyoun Kima
aDepartment of Civil and Environmental Engineering, Yonsei University, Seoul 03722, Republic of Korea
bEco Lab Center, SK Ecoplant Co. Ltd., Seoul 03143, Republic of Korea
cGreen and Sustainable Materials R&D Department, Korea Institute of Industrial Technology (KITECH), Cheonan 31056, Republic of Korea
Corresponding author: Sang-Hyoun Kim
Abstract
This study aimed to mitigate the instability in the start-up and continuous performance of dark fermentative biohydrogen production using heat-treated sludge by the addition of an exogenous H2-producing strain. Continuous fermentation augmented with Clostridium butyricum showed the highest average biohydrogen production rate (HPR) as 50.35 ± 2.56 and 58.57 ± 5.03 L/L-d with H2-producing butyric and acetic acid pathways, whereas the fermenters without bioaugmentation showed the termination of biohydrogen production in 3 days of continuous operation with non H2-producing lactic acid pathway and H2-consuming propionic acid pathway. The bioaugmentation blocked the growth of the competitors for hexose such as Streptococcus, Lactobacillus and Megasphaera, and provided H2-producer dominated microbiome with not only Clostridium butyricum, but also Clostridium puniceum and Clostridium neuense originated from heat-treated sludge. Bioaugmentation of a H2-producing strain would be a reliable dissemination strategy for dark fermentative biohydrogen production by minimizing the influence of seed sludge population.
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