한빛사논문
- 조회356
Sunkyung Choia,1, Eun-Mi Kimb,1, Seung-Yeon Kima,b,1, Yeongsoo Choia, Seri Choib, Namjoon Choa, Han-Jin Parkb, Kee K. Kima
aDepartment of Biochemistry, Chungnam National University, Daejeon, 34134, Republic of Korea
bDepartment of Predictive Toxicology, Korea Institute of Toxicology, Daejeon, 34114, Republic of Korea
1These authors contributed equally to this work.
Corresponding author: Kee K. Kim
Abstract
Exposure to atmospheric particulate matter (PM) increases morbidity and mortality in respiratory diseases by causing various adverse health effects; however, the effects of PM exposure on cellular stress under virus-infected conditions remain unclear. The effects of PM under 10 μm (PM10) and diesel PM (DPM) on respiratory syncytial virus (RSV) infection were investigated in human two-dimensional lung epithelial cells and human three-dimensional lung organoids mimicking the lung tissue. We evaluated the formation of stress granules, which are important in cellular adaptation to various stress conditions. Furthermore, we investigated the effects of repeated exposure to PM10 and DPM on DNA damage and cell death during viral infection. PM10 and DPM did not cause stress granule formation in the absence of RSV infection but drastically increased stress granule formation and signal transduction during RSV infection in human lung epithelial cells and human lung organoids. Further, repeated exposure to PM10 and DPM caused cell death by severely damaging DNA under RSV infection conditions. Thus, PM10 and DPM induce severe lung toxicity under stress conditions, such as viral infection, suggesting that the effects of PMs under various stressful conditions should be examined to accurately predict the lung toxicity of PM.
논문정보
- Research article
- 2022년 12월 (BRIC 등록일 2023-01-02)
- 국내 연구진
- 생명과학 > 분자생물학
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