한빛사논문
- 조회443
Hanjun Hwangbo, Hyeongjin Lee, Eun-Ju Jin, Yunju Jo, Jigyeong Son, Han Min Woo,* Dongryeol Ryu,* and Geun Hyung Kim*
H. Hwangbo, H. Lee, G. H. Kim
Department of Biomechatronic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan University (SKKU) Suwon 16419, Republic of Korea
E.-J. Jin, Y. Jo, D. Ryu
Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon 16419, Republic of Korea
J. Son, H. M. Woo
Department of Food Science and Biotechnology, College of Biotechnology and Bioengineering, Sungkyunkwan University, Suwon 16419, Republic of Korea
G. H. Kim
Biomedical Institute for Convergence at SKKU (BICS), Sungkyunkwan University, Suwon 16419, Republic of Korea
H. H. and H. L. contributed equally to this work.
*CORRESPONDING AUTHORS: Han Min Woo, Dongryeol Ryu, Geun Hyung Kim
Abstract
Tissue engineering strategies using cell-laden constructs have shown promising results in the treatment of various types of damaged tissues. However, inadequate oxygen delivery to the macroscale 3D cell-constructs for regenerating skeletal muscle tissue has remained a multiplex issue owing to the pivotal factors including cell metabolism and several regulatory intercellular pathways that eventually influence various cellular activities and determines cell phenotype. To overcome this issue, a photosynthetic cyanobacterium (Synechococcus elongatus) is employed in a methacrylated gelatin bioink. Furthermore, to effectively induce cell alignment in the bioink, in situ electric field stimulation is used in a bioprinting system to fabricate cell-laden scaffolds for regenerating skeletal muscle tissue. Owing to the synergistic effects of the bioactive microenvironment that rescues cells from hypoxic conditions and activations of voltage-gated ion channels, highly aligned, multi-nucleated myofibers are obtained as well as significant upregulation (7–10-fold) of myogenic-related genes compared with conventionally prepared cell-constructs. In addition, in vivo studies using a mouse volumetric muscle loss model demonstrate considerable restoration of muscle functionality and regeneration.
논문정보
- Research article
- 2022년 12월 (BRIC 등록일 2022-12-29)
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