한빛사논문
- 조회523
한국생명공학연구원
Seung Yong Shin1,2, Yuri Choi3, Sang-Gyu Kim3, Su-Jin Park1,4, Ji-Sun Park1, Ki-Beom Moon1, Hyun-Soon Kim1,4, Jae Heung Jeon1, Hye Sun Cho1,4, Hyo-Jun Lee1,2,5
1Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Korea
2Department of Functional Genomics, KRIBB School of Bioscience, University of Science and Technology, Daejeon 34113, Korea
3Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 34141, Korea
4Department of Biosystems and Bioengineering, KRIBB School of Biotechnology, University of Science and Technology, Daejeon 34113, Korea
5Department of Biological Sciences, Sungkyunkwan University, Suwon 16419, Korea
Corresponding author: Hyo-Jun Lee
Abstract
Plant cells in damaged tissue can be reprogrammed to acquire pluripotency and induce callus formation. However, in the aboveground organs of many species, somatic cells that are distal to the wound site become less sensitive to auxin-induced callus formation, suggesting the existence of repressive regulatory mechanisms that are largely unknown. Here we reveal that submergence-induced ethylene signals promote callus formation by releasing post-transcriptional silencing of auxin receptor transcripts in non-wounded regions. We determined that short-term submergence of intact seedlings induces auxin-mediated cell dedifferentiation across the entirety of Arabidopsis thaliana explants. The constitutive triple response 1-1 (ctr1-1) mutation induced callus formation in explants without submergence, suggesting that ethylene facilitates cell dedifferentiation. We show that ETHYLENE-INSENSITIVE 2 (EIN2) post-transcriptionally regulates the abundance of transcripts for auxin receptor genes by facilitating microRNA393 degradation. Submergence-induced calli in non-wounded regions were suitable for shoot regeneration, similar to those near the wound site. We also observed submergence-promoted callus formation in Chinese cabbage (Brassica rapa), indicating that this may be a conserved mechanism in other species. Our study identifies previously unknown regulatory mechanisms by which ethylene promotes cell dedifferentiation and provides a new approach for boosting callus induction efficiency in shoot explants.
논문정보
- Research article
- 2022년 12월 (BRIC 등록일 2022-12-26)
- 국내 연구진
- 생명과학 > 식물학
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