한빛사논문
- 조회438
Gi Uk Jeong1,13, Hyung-Jun Kwon2,3,13, Wern Hann Ng4,5,6, Xiang Liu4,5,6, Hyun Woo Moon 1, Gun Young Yoon 1, Hye Jin Shin1,12, In-Chul Lee2,3, Zheng Lung Ling7,8, Alanna G. Spiteri7,8, Nicholas J. C. King 4,7,8,9,10, Adam Taylor4,5,6, Ji Soo Chae11, Chonsaeng Kim1, Dae-Gyun Ahn 1, Kyun-Do Kim1, Young Bae Ryu2, Seong-Jun Kim 1, Suresh Mahalingam 4,5,6,14 & Young-Chan Kwon 1,14
1Department of Convergent Research of Emerging Virus Infection, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.
2Department of Functional Biomaterial Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup 56212, Republic of Korea.
3Center for Companion Animal New Drug Development, Jeonbuk Branch, Korea Institute of Toxicology, Jeongeup 53212, Republic of Korea.
4Emerging Viruses, Inflammation and Therapeutics Group, Menzies Health Institute Queensland, Griffith University, Gold Coast, QLD, Australia.
5Global Virus Network(GVN) Centre of Excellence in Arboviruses, Griffith University, Gold Coast, QLD, Australia.
6School of Pharmacy and Medical Sciences, Griffith University, Gold Coast, QLD, Australia.
7Viral Immunopathology Laboratory, The Charles Perkins Centre, The University of Sydney, Sydney, NSW 2006, Australia.
8School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006, Australia.
9Sydney Institute for Infectious Diseases, The University of Sydney, Sydney, NSW 2006, Australia.
10Sydney Nano, The University of Sydney, Sydney, NSW 2006, Australia. 11Department of Life Sciences, PerkinElmer, Seoul 08380, Republic of Korea.
12Present address: Department of Microbiology, Chungnam National University School of Medicine, Daejeon 35015, Republic of Korea.
13These authors contributed equally: Gi Uk Jeong, Hyung-Jun Kwon.
14These authors jointly supervised this work: Suresh Mahalingam, Young-Chan Kwon
Corresponding author : Correspondence to Young-Chan Kwon.
Abstract
Although ocular manifestations are reported in patients with COVID-19, consensus on ocular tropism of SARS-CoV-2 is lacking. Here, we infect K18-hACE2 transgenic mice with SARS-CoV-2 using various routes. We observe ocular manifestation and retinal inflammation with production of pro-inflammatory cytokines in the eyes of intranasally (IN)-infected mice. Intratracheal (IT) infection results in dissemination of the virus from the lungs to the brain and eyes via trigeminal and optic nerves. Ocular and neuronal invasions are confirmed using intracerebral (IC) infection. Notably, the eye-dropped (ED) virus does not cause lung infection and becomes undetectable with time. Ocular and neurotropic distribution of the virus in vivo is evident in fluorescence imaging with an infectious clone of SARS-CoV-2-mCherry. The ocular tropic and neuroinvasive characteristics of SARS-CoV-2 are confirmed in wild-type Syrian hamsters. Our data can improve the understanding regarding viral transmission and clinical characteristics of SARS-CoV-2 and help in improving COVID-19 control procedures.
논문정보
- Research article
- 2022년 12월 (BRIC 등록일 2022-12-21)
- 국내(교신)+국외 연구진
- 생명과학 > 미생물학
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