한빛사논문
Ji-Eun Jeonga,b,1, Sang-Soo Hanc,1, Hye-Eun Shima, Woojin Kimd, Byoung-Seok Leed, Yong-Jin Kime, Sun-Woong Kanga,b
aResearch Group for Biomimetic Advanced Technology, Korea Institute of Toxicology, Daejeon 34114, Republic of Korea
bDepartment of Human and Environmental Toxicology, University of Science and Technology, Daejeon 34114, Republic of Korea
cAdvanced Defense Science & Technology Research Institute, Agency for Defense Development, Daejeon 34186, Republic of Korea
dDepartment of Toxicological Evaluation and Research, Korea Institute of Toxicology, Daejeon, 34114, Republic of Korea
eAMOREPACIFIC APAC R&I, Singapore 138667, Singapore
1These authors contributed equally to this work as co-first authors.
Corresponding authors: Yong-Jin Kim, Sun-Woong Kang
Abstract
3D spheroids, which have the potential to bridge the gap between 2D cell culture and native tissue, are used as tissue models in many applications, particularly in cancer, stem cell, and pharmaceutical research. A considerable amount of effort has focused on the development of more relevant physiological models. However, spheroids still have limitations in that they cannot replicate the components and structure of the ECM in the natural environment. In this study, we proposed new concept of scaffold-based techniques for the generation of spheroids. Spheroids were successfully generated by single cell or small number of aggregated cells between HA particles. The size of each spheroid was uniform, a necrotic core didn't form, and the system showed high viability. The expression levels of the proteins and genes required to maintain cell-specific functions increased. Thus, our system provides more physiologically relevant models and could be applied to regenerative medicine or drug screening.
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