한빛사논문
- 조회427
Hyo Gyeong Lee,‡a Seokbeom Roh,‡b,c Hyun Jung Kim,b,d Seokho Kim,e Yoochan Hong,*d Gyudo Lee *b,c and Ok Hee Jeon*a
a Department of Biomedical Sciences, Korea University College of Medicine, Seoul 02841, Republic of Korea.
b Department of Biotechnology and Bioinformatics, Korea University, Sejong 30019, Republic of Korea.
c Interdisciplinary Graduate Program for Artificial Intelligence Smart Convergence Technology, Korea University, Sejong 30019, Republic of Korea
d Department of Medical Device, Korea Institute of Machinery and Materials (KIMM), Daegu 42994, Republic of Korea.
e Animal Biotechnology Division, National Institute of Animal Science, Rural Development Administration, Wanju 55365, Republic of Korea
‡ H. G. Lee and S. Roh contributed equally to this work
*Corresponding authors : Yoochan Hong, Gyudo Lee and Ok Hee Jeon
Abstract
Cells secrete extracellular vesicles (EVs) carrying cell-of-origin markers to communicate with surrounding cells. EVs regulate physiological processes ranging from intercellular signaling to waste management. However, when senescent cells (SnCs) secrete EVs, the EVs, which are newly regarded as senescence-associated secretory phenotype (SASP) factors, can evoke inflammation, senescence induction, and metabolic disorders in neighboring cells. Unlike other soluble SASP factors, the biophysical properties of EVs, including small EVs (sEVs), derived from SnCs have not yet been investigated. In this study, sEVs were extracted from a human IMR90 lung fibroblast in vitro senescence model. Their biomechanical properties were mapped using atomic force microscopy-based quantitative nanomechanical techniques, surface potential microscopy, and Raman spectroscopy. The surfaces of sEVs derived from SnCs are slightly stiffer but their cores are softer than those of sEVs secreted from non-senescent cells (non-SnCs). This inversely proportional relationship between deformation and stiffness, attributed to a decrease in the concentration of genetic and protein materials inside the vesicles and the adsorption of positively charged SASP factors onto the vesicle surfaces, respectively, was found to be a peculiar characteristic of SnC-derived sEVs. Our results demonstrate that the biomechanical properties of SnC-derived sEVs differ from those of non-SnC-derived sEVs and provide insight into the mechanisms underlying their formation and composition.
논문정보
- Research article
- 2022년 11월 (BRIC 등록일 2022-12-02)
- 국내 연구진
- 생명과학 > 노화/암생물학
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