오원찬 (University of Colorado School of Medicine) | 한빛사논문 > 한빛사

한빛사논문

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University of Colorado School of Medicine

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Neuron, Published: November 16, 2022 | https://doi.org/10.1016/j.neuron.2022.11.002 Dually innervated dendritic spines develop in the absence of excitatory activity and resist plasticity through tonic inhibitory crosstalk

Mason S. Kleinjan,1 William C. Buchta,1 Roberto Ogelman,1 In-Wook Hwang,1 Masaaki Kuwajima,3 Dusten D. Hubbard,3 Dean J. Kareemo,1 Olga Prikhodko,1 Samantha L. Olah,1 Luis E. Gomez Wulschner,1 Wickliffe C. Abraham,4 Santos J. Franco,2 Kristen M. Harris,3 Won Chan Oh,1,* and Matthew J. Kennedy1,5,*

1Department of Pharmacology, University of Colorado School of Medicine, Aurora, CO 80045, USA
2Department of Pediatrics, University of Colorado School of Medicine, Aurora, CO 80045, USA
3Department of Neuroscience, University of Texas at Austin, Austin, TX 78712, USA
4Department of Psychology, Brain Health Research Centre, University of Otago, Dunedin, New Zealand
5Lead contact

*Correspondence: Won Chan Oh and Matthew J. Kennedy

Abstract

Dendritic spines can be directly connected to both inhibitory and excitatory presynaptic terminals, resulting in nanometer-scale proximity of opposing synaptic functions. While dually innervated spines (DiSs) are observed throughout the central nervous system, their developmental timeline and functional properties remain uncharacterized. Here we used a combination of serial section electron microscopy, live imaging, and local synapse activity manipulations to investigate DiS development and function in rodent hippocampus. Dual innervation occurred early in development, even on spines where the excitatory input was locally silenced. Synaptic NMDA receptor currents were selectively reduced at DiSs through tonic GABA_B receptor signaling. Accordingly, spine enlargement normally associated with long-term potentiation on singly innervated spines (SiSs) was blocked at DiSs. Silencing somatostatin interneurons or pharmacologically blocking GABA_BRs restored NMDA receptor function and structural plasticity to levels comparable to neighboring SiSs. Thus, hippocampal DiSs are stable structures where function and plasticity are potently regulated by nanometer-scale GABAergic signaling.

논문정보

형식Research article
게재일2022년 11월 (BRIC 등록일 2022-11-17)
연구진국외 연구진
분야 생명과학 > 신경생물학

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