한빛사논문
- 조회553
Won-Seok Lee # 1 2 3, Ji-Hye Kang # 4 2, Jung-Hwan Lee 4 2 5 6 3, Yoo Sung Kim 1, Jongmin Joseph Kim 1, Han-Sem Kim 2, Hae-Won Kim 4 2 5 6 3, Ueon Sang Shin 7 8, Bo-Eun Yoon 9 10 11
1Department of Molecular Biology, Dankook University, Cheonan, 31116, Republic of Korea.
2Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, 31116, Republic of Korea.
3Mechanobiology Dental Medicine Research Center, Cheonan, 31116, Republic of Korea.
4Department of Nanobiomedical Science, BK21 FOUR NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan, 31116, Republic of Korea.
5Department of Biomaterials Science, College of Dentistry, Dankook University, Cheonan, 31116, Republic of Korea.
6Dental Medicine Innovation Centre, UCL Eastman-Korea, Dankook University, Cheonan, 31116, Republic of Korea.
7Department of Nanobiomedical Science, BK21 FOUR NBM Global Research Center for Regenerative Medicine, Dankook University, Cheonan, 31116, Republic of Korea.
8Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, 31116, Republic of Korea.
9Department of Molecular Biology, Dankook University, Cheonan, 31116, Republic of Korea.
10Institute of Tissue Regeneration Engineering (ITREN), Dankook University, Cheonan, 31116, Republic of Korea.
11Mechanobiology Dental Medicine Research Center, Cheonan, 31116, Republic of Korea.
#Contributed equally.
Corresponding authors: Correspondence to Ueon Sang Shin or Bo-Eun Yoon.
Abstract
Background
Astrocyte is a key regulator of neuronal activity and excitatory/inhibitory balance via gliotransmission. Recently, gliotransmission has been identified as a novel target for neurological diseases. However, using the properties of nanomaterials to modulate gliotransmission has not been uncovered.
Results
We prepared non-invasive CNT platforms for cells with different nanotopography and properties such as hydrophilicity and conductivity. Using CNT platforms, we investigated the effect of CNT on astrocyte functions participating in synaptic transmission by releasing gliotransmitters. Astrocytes on CNT platforms showed improved cell adhesion and proliferation with upregulated integrin and GFAP expression. In addition, intracellular GABA and glutamate in astrocytes were augmented on CNT platforms. We also demonstrated that gliotransmitters in brain slices were increased by ex vivo incubation with CNT. Additionally, intracellular resting Ca2+ level, which is important for gliotransmission, was also increased via TRPV1 on CNT platforms.
Conclusion
CNT can improve astrocyte function including adhesion, proliferation and gliotransmission by increasing resting Ca2+ level. Therefore, our study suggests that CNT would be utilized as a new therapeutic platform for central nervous system diseases by modulating gliotransmission.
논문정보
- Research article
- 2022년 08월 (BRIC 등록일 2022-11-03)
- 국내 연구진
- 생명과학 > 신경생물학
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