한빛사논문
- 조회429
Moon-Jung Yong†,a,b, Byunghwa Kang†,b, Un Yang†,a,b, Seung Soo Ohb*and Jung Ho Jea,b**
aX-ray Imaging Center, bDepartment of Materials Science and Engineering, Pohang University of Science and Technology (POSTECH), 77 Cheongam-Ro, Nam-Gu, Pohang, Gyeongbuk 37673, South Korea
†These authors contributed equally to this work.
Corresponding Authors: Seung Soo Oh, Jung Ho Je
Abstract
Spatiotemporal pH monitoring of single living cells across rigid cell and organelle membranes has been challenging, despite its significance in understanding cellular heterogeneity. Here, we developed a mechanically robust yet tolerably thin nanowire waveguide that enables in situ monitoring of pH dynamics at desired cellular compartments via direct optical communication. By chemically labeling fluorescein at one end of a poly(vinylbenzyl azide) nanowire, we continuously monitored pH variations of different compartments inside a living cell, successfully observing organelle-exclusive pH homeostasis and stimuli-selective pH regulations. Importantly, it was demonstrated for the first time that, during the mammalian cell cycle, the nucleus displays pH homeostasis in interphase but a tidal pH curve in the mitotic phase, implying the existence of independent pH-regulating activities by the nuclear envelope. The rapid and accurate local pH-reporting capability of our nanowire waveguide would be highly valuable for investigating cellular behaviors under diverse biological situations in living cells.
논문정보
- Research article
- 2022년 07월 (BRIC 등록일 2022-11-01)
- 국내 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
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