한빛사논문
- 조회419
Tin Hoang Trung Chau 1, Anh Duc Nguyen 1, Eun Yeol Lee *
Department of Chemical Engineering (BK21 FOUR Integrated Engineering Program), Kyung Hee University, Yongin-si, Gyeonggi-do 17104, South Korea
* Corresponding author.
1 These authors contributed equally to this work.
Abstract
Methylotuvimicrobium alcaliphilum 20Z recombinant strain co-utilizing methane and xylose from anthropogenic activities and lignocellulose biomass is a promising cell factory platform. In this study, the production of (R)-3-hydroxybutyrate and poly (3-hydroxybutyrate) in M. alcaliphilum 20Z was demonstrated. The production of (R)-3-hydroxybutyrate was optimized by introducing additional thioesterase, and a tunable genetic module. The final recombinant strain produced the highest titer of 334.52 ± 2 mg/L (R)-3-hydroxybutyrate (yield of 1,853 ± 429 mg/g dry cell weight). The poly (3-hydroxybutyrate) yielded 1.29 ± 0.08% (w/w) from methane and xylose in one-stage cultivation. Moreover, the study demonstrated the importance of pathway reversibility as an effective design strategy for balancing the driving force and intermediate accumulation. This is the first demonstration of the production of biodegradable poly (3-hydroxybutyrate) from methane in type I methanotrophs, which is a key step toward sustainable biomanufacturing and carbon–neutral society.
논문정보
- Research article
- 2022년 11월 (BRIC 등록일 2022-10-12)
- 국내 연구진
- 생명과학 > 생물공학
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