한빛사논문
- 조회265
Hojae Lee, Joohyouck Park, Nayoung Kim, Wongu Youn, Gyeongwon Yun, Sang Yeong Han, Duc Tai Nguyen, and Insung S. Choi*
H. Lee, J. Park, N. Kim, W. Youn, G. Yun, S. Y. Han, D. T. Nguyen, I. S. Choi
Center for Cell-Encapsulation Research Department of Chemistry KAIST Daejeon 34141, South Korea
*Corresponding author.
Abstract
Cell-in-shell biohybrid structures, synthesized by encapsulating individual living cells with exogenous materials, have emerged as exciting functional entities for engineered living materials, with emergent properties outside the scope of biochemical modifications. Artificial exoskeletons have, to date, provided physicochemical shelters to the cells inside in the first stage of technological development, and further advances in the field demand catalytically empowered, cellular hybrid systems that augment the biological functions of cells and even introduce completely new functions to the cells. This work describes a facile and generalizable strategy for empowering living cells with extrinsic catalytic capability through nanoencapsulation of living cells with a supramolecular metal–organic complex of Fe3+ and benzene-1,3,5-tricarboxylic acid (BTC). A series of enzymes are embedded in situ, without loss of catalytic activity, in the Fe3+-BTC shells, not to mention the superior characteristics of cytocompatible and rapid shell-forming processes. The nanoshell enhances the catalytic efficiency of multienzymatic cascade reactions by confining reaction intermediates to its internal voids and the nanoencapsulated cells acquire exogenous biochemical functions, including enzymatic cleavage of lethal octyl-β-d-glucopyranoside into d-glucose, with autonomous cytoprotection. The system will provide a versatile, nanoarchitectonic tool for interfacing biological cells with functional materials, especially for catalytic bioempowerment of living cells.
논문정보
- Research article
- 2022년 07월 (BRIC 등록일 2022-10-27)
- 국내 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
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