한빛사논문
- 조회385
POSTECH
Xiao Liu a,b,1, Takeru Yamazaki c,1, Haw-Young Kwon a, Satoshi Arai c,*, Young-Tae Chang a,b,*
a Center for Self-assembly and Complexity, Institute for Basic Science (IBS), Pohang, Gyeongbuk, 37673, South Korea
b Department of Chemistry, Pohang University of Science and Technology (POSTECH), Pohang, Gyeongbuk, 37673, South Korea
c WPI Nano Life Science Institute, Kanazawa University, Kakuma-machi, Kanazawa, 920-1192, Japan
* Corresponding author.
1 These authors contributed equally
Abstract
Intracellular micro-temperature is closely related to cellular processes. Such local temperature inside cells can be measured by fluorescent thermometers, which are a series of fluorescent materials that convert the temperature information to detectable fluorescence signals. To investigate the intracellular temperature fluctuation in various organelles, it is essential to develop site-specific organelle thermometers. In this study, we develop a new series of fluorescent thermometers, Thermo Greens (TGs), to visualize the temperature change in almost all typical organelles. Through fluorescence lifetime-based cell imaging, it was proven that TGs allow the organelle-specific monitoring of temperature gradients created by external heating. The fluorescence lifetime-based thermometry shows that each organelle experiences a distinct temperature increment which depends on the distance away from the heat source. TGs are further demonstrated in the quantitative imaging of heat production at different organelles such as mitochondria and endoplasmic reticulum in brown adipocytes. To date, TGs are the first palette batch of small molecular fluorescent thermometers that can cover almost all typical organelles. These findings can inspire the development of new fluorescent thermometers and enhance the understanding of thermal biology in the future.
논문정보
- Research article
- 2022년 08월 (BRIC 등록일 2022-09-05)
- 국내(교신)+국외 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
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