한빛사논문
- 조회397
Min Jung Kwon1,8, Yeojin Seo1,2, Hana Cho3, Hyung Soon Kim1,2, Young Joo Oh1,2, Simay Genişcan1,2, Minjae Kim4, Hee Hwan Park1,2, Eun-Hye Joe1,2,5,6, Myung-Hee Kwon4, Han Chang Kang3, Byung Gon Kim1,2,6,7,8*
1. Department of Brain Science, Ajou University Graduate School of Medicine, Suwon, 16499, Republic of Korea.
2. Neuroscience Graduate Program, Department of Biomedical Sciences, Ajou University Graduate School of Medicine, Suwon, 16499, Republic of Korea.
3. Department of Pharmacy, College of Pharmacy, The Catholic University of Korea, Bucheon, 14662, Republic of Korea.
4. Department of Microbiology, Ajou University School of Medicine, Suwon 16499, Republic of Korea.
5. Department of Pharmacology, Ajou University School of Medicine, Suwon, 16499, Republic of Korea.
6. Center for Convergence Research of Neurological Disorders, Ajou University School of Medicine, Suwon, 16499, Republic of Korea.
7. Department of Neurology, Ajou University School of Medicine, Suwon, 16499, Republic of Korea.
8. AI-Superconvergence KIURI Translational Research Center, Suwon, 16499, Republic of Korea.
*Corresponding author.
Abstract
Preconditioning nerve injury enhances axonal regeneration of dorsal root ganglia (DRG) neurons in part by driving pro-regenerative perineuronal macrophage activation. How these macrophages influence the neuronal capacity of axon regeneration remains elusive. We report that oncomodulin (ONCM) is produced from the regeneration-associated macrophages and strongly influences regeneration of DRG sensory axons. We also attempted to promote sensory axon regeneration by nanogel-mediated delivery of ONCM to DRGs.
Methods: In vitro neuron-macrophage interaction model and preconditioning sciatic nerve injury were used to verify the necessity of ONCM in preconditioning injury-induced neurite outgrowth. We developed a nanogel-mediated delivery system in which electrostatic encapsulation of ONCM by a reducible epsilon-poly(L-lysine)-nanogel (REPL-NG) enabled a controlled release of ONCM.
Results: Sciatic nerve injury upregulated ONCM in DRG macrophages. ONCM in macrophages was necessary to produce pro-regenerative macrophages in the in vitro model of neuron-macrophage interaction and played an essential role in preconditioning-induced neurite outgrowth. ONCM increased neurite outgrowth in cultured DRG neurons by activating a distinct gene set, particularly neuropeptide-related genes. Increasing extracellularly secreted ONCM in DRGs sufficiently enhanced the capacity of neurite outgrowth. Intraganglionic injection of REPL-NG/ONCM complex allowed sustained ONCM activity in DRG tissue and achieved a remarkable long-range regeneration of dorsal column sensory axons beyond spinal cord lesion.
Conclusion: NG-mediated ONCM delivery could be exploited as a therapeutic strategy for promoting sensory axon regeneration following spinal cord injury.
논문정보
- Research article
- 2022년 08월 (BRIC 등록일 2022-09-02)
- 국내 연구진
- 의약학 > 신경의학
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