한빛사논문
- 조회423
Aseer Intisar, Hyun Young Shin, Woon-Hae Kim, Hyun Gyu Kang, Min Young Kim, Yu Seon Kim, Youngjun Cho, Yun Jeoung Mo, Heejin Lim, Sanghoon Lee, Q. Richard Lu, Yun-Il Lee, and Minseok S. Kim*
A. Intisar, H. G. Kang, M. Y. Kim, H. Lim, M. S. Kim
Department of New Biology DGIST Daegu 42988, Republic of Korea
H. Y. Shin, W.-H. Kim, M. S. Kim
CTCELLS Corp. Daegu 42988, Republic of Korea
H. Y. Shin
SBCure Corp. Daegu 43017, Republic of Korea
Y. S. Kim, Y. J. Mo, Y.-I. Lee
Well Aging Research Center DGIST Daegu 42988, Republic of Korea
Y. Cho, S. Lee
Department of Robotics and Mechatronics Engineering DGIST Daegu 42988, Republic of Korea
Q. R. Lu
Department of Pediatrics Cincinnati Children’s Hospital Medical Center Cincinnati, OH 45229, USA
M. S. Kim
Translational Responsive Medicine Center (TRMC) DGIST Daegu 42988, Republic of Korea
M. S. Kim
New Biology Research Center (NBRC) DGIST Daegu 42988, Republic of Korea
*Corresponding author.
Abstract
Although many efforts are undertaken to treat peripheral demyelinating neuropathies based on biochemical interventions, unfortunately, there is no approved treatment yet. Furthermore, previous studies have not shown improvement of the myelin membrane at the biomolecular level. Here, an electroceutical treatment is introduced as a biophysical intervention to treat Charcot-Marie-Tooth (CMT) disease—the most prevalent peripheral demyelinating neuropathy worldwide—using a mouse model. The specific electrical stimulation (ES) condition (50 mV mm−1, 20 Hz, 1 h) for optimal myelination is found via an in vitro ES screening system, and its promyelinating effect is validated with ex vivo dorsal root ganglion model. Biomolecular investigation via time-of-flight secondary ion mass spectrometry shows that ES ameliorates distribution abnormalities of peripheral myelin protein 22 and cholesterol in the myelin membrane, revealing the restoration of myelin membrane integrity. ES intervention in vivo via flexible implantable electrodes shows not only gradual rehabilitation of mouse behavioral phenotypes (balance and endurance), but also restored myelin thickness, compactness, and membrane integrity. This study demonstrates, for the first time, that an electroceutical approach with the optimal ES condition has the potential to treat CMT disease and restore impaired myelin membrane integrity, shifting the paradigm toward practical interventions for peripheral demyelinating neuropathies.
논문정보
- Research article
- 2022년 08월 (BRIC 등록일 2022-09-05)
- 국내(교신)+국외 연구진
- 바이오·의료융합 > 바이오센싱 및 나노바이오물질
댓글 작성 로그인
팝업 닫기관련 링크
연구자 키워드
관련분야 연구자보기
관련분야 논문보기
해당논문 저자보기
