이윤상 (서울대학교)

한빛사논문

조회388

서울대학교

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ACS Nano, Published online 9 August 2022 | https://doi.org/10.1021/acsnano.2c03075 Circulation Time-Optimized Albumin Nanoplatform for Quantitative Visualization of Lung Metastasis via Targeting of Macrophages

Hyewon Chung,^1,2,‡ Ji Yong Park,^3,4,5,6‡ Kyuwan Kim,^4,5 Ran Ji Yoo,^4,5 Minseok Suh,⁷ Gyo Jeong Gu,¹ Jin Sil Kim,⁴ Tae Hyeon Choi,^4,7 Jung Woo Byun,⁴ Young Wook Ju,⁸ Wonshik Han,⁸ Han Suk Ryu,⁹ Gehoon Chung,^6,10 Do Won Hwang,^4,11 Yujin Kim,³ Hye-Ryun Kang,³ Yi Rang Na,¹²Hongyoon Choi,⁴ Hyung-Jun Im,^7,13 Yun-Sang Lee,^3,4,5,* Seung Hyeok Seok^1,3,*

¹Macrophage Lab, Department of Microbiology and Immunology, and Institute of Endemic Disease, Seoul National University College of Medicine, Seoul 03080, Republic of Korea
²Bio-MAX Institute, Seoul National University, Seoul 03080, Republic of Korea
³Department of Biomedical Sciences, Seoul National University Graduate School, Seoul 03080, Republic of Korea
⁴Department of Nuclear Medicine, Seoul National University Hospital, Seoul 03080, Republic of Korea
⁵Cancer Research Institute, Seoul National University, Seoul 03080, Republic of Korea
⁶Dental Research Institute, Seoul National University, Seoul 03080, Republic of Korea
⁷Department of Molecular Medicine and Biopharmaceutical Sciences, Graduate School of Convergence Science and Technology, Seoul National University; Seoul, Republic of Korea
⁸Department of Surgery and Cancer Research Institute, Seoul National University College of Medicine, Seoul 03080, Republic of Korea
⁹Department of Pathology, Seoul National University College of Medicine, Seoul 03080, Republic of Korea
¹⁰Department of Oral Physiology, Seoul National University, School of Dentistry, Seoul 03080, Republic of Korea
¹¹Research and Development Center, THERABEST, Co. Ltd., Seoul, Korea
¹²Transdisciplinary Department of Medicine and Advanced Technology, Seoul National University Hospital, Seoul 03080, Republic of Korea
¹³Research Institute for Convergence Science, Seoul National University; Seoul, 08826, Republic of Korea
^‡These authors contributed equally.

^*Corresponding author.

Abstract

The development of molecular imaging probes to identify key cellular changes within lung metastases may lead to noninvasive detection of metastatic lesions in the lung. In this study, we constructed a macrophage-targeted clickable albumin nanoplatform (CAN) decorated with mannose as the targeting ligand using a click reaction to maintain the intrinsic properties of albumin in vivo. We also modified the number of mannose molecules on the CAN and found that mannosylated serum albumin (MSA) harboring six molecules of mannose displayed favorable pharmacokinetics that allowed high-contrast imaging of the lung, rendering it suitable for in vivo visualization of lung metastases. Due to the optimized control of functionalization and surface modification, MSA enhanced blood circulation time and active/passive targeting abilities and was specifically incorporated by mannose receptor (CD206)-expressing macrophages in the metastatic lung. Moreover, extensive in vivo imaging studies using single-photon emission computed tomography (SPECT)/CT and positron emission tomography (PET) revealed that blood circulation of time-optimized MSA can be used to discern metastatic lesions, with a strong correlation between its signal and metastatic burden in the lung.

논문정보

형식Research article
게재일2022년 08월 (BRIC 등록일 2022-08-19)
연구진국내 연구진
분야 의약학 > 면역의학

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