한빛사논문
- 조회760
Soon Kyu Leea*, JooYeon Jhunb,c*, Seung Yoon Leeb,c, Sukjung Choid, Sun Shim Choid, Myeong Soo Parke, SeonYoung Leeb, Keun-Hyung Chob,c, A Ram Leeb,c, Joseph Ahnf, Ho Joong Choif, Young Kyoung Youf, Pil Soo Sungg, Jeong Won Jangg, Si Hyun Baeh, Seung Kew Yoong, Mi-La Chob,i, and Jong Young Choig
aDivision of gastroenterology and hepatology, Department of Internal Medicine, Incheon St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea; bThe Rheumatism Research Center, Catholic Research Institute of Medical Science, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea; cDepartment of Biomedicine & Health Sciences, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea; dDivision of Biomedical Convergence, College of Biomedical Science, Institute of Bioscience & Biotechnology, Kangwon National University, Chuncheon, Korea; eResearch Center, BIFIDO Co., Ltd, Hongcheon, Korea; fDepartment of Surgery, Seoul St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea; gDivision of gastroenterology and hepatology, Department of Internal Medicine, Seoul St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea; hDivision of gastroenterology and hepatology, Department of Internal Medicine, Eunpyeong St. Mary’s Hospital, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea; iDepartment of Medical Lifescience, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea
*SKL and JYJ contributed equally to this study.
CONTACT Mi-La Cho, Jong Young Choi
Abstract
This study aimed to document the functional microbiome affecting immune homeostasis in long-term post-liver transplantation (LT) patients. We compared the frequency of regulatory T (Treg) and T helper 17 (Th17) cells in the blood and fecal microbiome of 27 LT patients to matched healthy controls (n = 20) using flow cytometry and 16S rRNA sequencing. Among the 27 LT patients, 22 patients ingested immunosuppressants (long-term post-LT group) and five were tolerant patients. The changes in Treg and Th17 cell proportion after treatment with distinct microbiomes in the long-term post-LT group were also examined in vitro. Changes in the identified functional microbiome and Treg cells in tolerant patients were evaluated. The mean time after LT of the included patients was 13.2 y. The gut microbiome of the long-term post-LT group showed lower alpha-diversity (P < .05) with distinct overall microbial composition (P = .001) compared to healthy controls. Among the 11 distinct bacterial genera in abundance, Faecalibacterium was the most decreased in the long-term post-LT group. The long-term post-LT group also demonstrated a decrease in Treg with an increase in Th17 cells, recovered by administration of F. prausnitzii and butyric acid in in vitro analysis. In tolerant patients, Faecalibacterium was marginally increased, coupled with an increase in Treg cells, compared to the long-term post-LT group. In conclusion, the long-term post-LT patients showed a decrease in functional microbiomes represented as Faecalibacterium. These findings provide potential biomarkers for assessing immune status and targets for improving immune homeostasis in LT patients.
논문정보
- Research article
- 2022년 08월 (BRIC 등록일 2022-08-18)
- 국내 연구진
- 생명과학 > 면역학
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